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PKM2 Antibody (703382RP555) in WB
Western blot was performed using PKM2 Recombinant Rabbit Monoclonal Antibody (3H4L19), Alexa Fluor™ Plus 555 (Product # 703382RP555) by loading 30 µg whole cell extracts of PANC-1 (Lane1), MCF-7 (Lane 2), SK-BR-3 (Lane 3) and Jurkat (Lane 4). The whole cell extracts were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001) and blocked with Blocker™ FL Fluorescent Blocking ... View More
Product Details
703382RP555
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
3H4L19
Immunogen
Peptides corresponding to human PKM2 [(aa476-aa493) (aa120-aa136)]
Conjugate
Excitation/Emission Max
558/572 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Amount
50 µg
Purification
Protein A
Storage buffer
proprietary buffer, pH 6.8
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTHBP; Cytosolic thyroid hormone-binding protein; cytosolic thyroid hormone-binding protein (EC 2.7.1.40); epididymis secretory protein Li 30; isozymes M1/M2; m2pk; OIP-3; OPA-interacting protein 3; p58; PK, muscle type; Pyruvate kinase 2/3; pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; Pyruvate kinase PKM; pyruvate kinase, muscle; similar to human pyruvate kinase M2; THBP1; Threonine-protein kinase PKM2; Thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; tu; Tumor M2-PK; Tyrosine-protein kinase PKM2; unnamed protein product
Gene Aliases: CTHBP; HEL-S-30; OIP3; p58; PK2; PK3; PKM; PKM2; TCB; THBP1
UniProt ID: (Human) P14618
Entrez Gene ID: (Human) 5315
nucleotide binding
magnesium ion binding
transcription coactivator activity
RNA binding
mRNA binding
catalytic activity
protein tyrosine kinase activity
pyruvate kinase activity
protein binding
ATP binding
kinase activity
transferase activity
MHC class II protein complex binding
potassium ion binding
histone kinase activity (H3-T11 specific)
protein homodimerization activity
cadherin binding
metal ion binding
glycolytic process
chromatin remodeling
regulation of translation
programmed cell death
cellular response to insulin stimulus
positive regulation of transcription from RNA polymerase II promoter
canonical glycolysis
positive regulation of sprouting angiogenesis
positive regulation of cytoplasmic translation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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