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FIGURE: 1 / 6
GM130 Antibody (703794) in ICC/IF
Knockdown of GM130 was achieved by transfecting HeLa cells with specific siRNA (Silencer® select Product # s5942). Immunofluorescence analysis was performed on HeLa cells (untransfected, panel a-d), transfected with GM130 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-GM130 Recombinant Rabbit Monoclonal Antibody (Product # 703794, 1:100 dilution), followed by Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (P... View More
Product Details
703794
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
10H5L5
Immunogen
Protein corresponding to human GM130 [aa69-aa157]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Mouse, Rat, Bovine.
Target Information
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 130 kDa cis-Golgi matrix protein; GM130; GM130 autoantigen; golgi autoantigen, golgin subfamily a, 2; Golgi matrix protein GM130; Golgin subfamily A member 2; Golgin-95; RP11-395P17.5; SY11 protein
Gene Aliases: DEDHMB; GM130; GOLGA2
UniProt ID: (Human) Q08379
Entrez Gene ID: (Human) 2801
protein glycosylation
ER to Golgi vesicle-mediated transport
microtubule nucleation
Golgi organization
centrosome cycle
asymmetric cell division
negative regulation of autophagy
protein transport
negative regulation of protein binding
spindle assembly
protein homotetramerization
cell division
positive regulation of protein glycosylation
Golgi ribbon formation
Golgi disassembly
spindle assembly involved in meiosis
mitotic spindle assembly
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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