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FIGURE: 1 / 4
CD199 (CCR9) Antibody (740187MP488) in ICC/IF
Immunofluorescence analysis of CD199 was performed using CD199 Recombinant Mouse Monoclonal Antibody (BBC3M4), Alexa Fluor™ Plus 488 (Product # 740187MP488) on 70-80% confluent MOLT-4 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, and then blocked with 2% BSA for 1 hour at room temperature. The cells were stained with CD199 Recombinant Mouse Monoclonal Antibody (BBC3M4), Alexa Fluor™ P... View More
Product Details
740187MP488
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
BBC3M4
Conjugate
Excitation/Emission Max
493/518 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Amount
50 µg
Purification
Protein A/G
Storage buffer
proprietary buffer, pH 6.8
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Applications Tested: This BBC3M4 antibody has been tested by flow cytometric analysis of MOLT-4 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to the chemokine receptor gene cluster region. Two alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C chemokine receptor type 9; C-C CKR-9; CCR9A; CCR9B; CDw199; CDw199 antigen; chemokine (C-C motif) receptor 9; G protein-coupled receptor; G protein-coupled receptor 28; G-protein coupled receptor 28; GPR-9-6; unnamed protein product
Gene Aliases: CC-CKR-9; CCR9; CDw199; GPR-9-6; GPR28
UniProt ID: (Human) P51686
Entrez Gene ID: (Human) 10803
CD8-positive, gamma-delta intraepithelial T cell differentiation
chemotaxis
immune response
cellular defense response
signal transduction
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
calcium-mediated signaling
cell chemotaxis
chemokine-mediated signaling pathway
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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