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FIGURE: 1 / 4
CD199 (CCR9) Antibody (740187M) in ICC/IF
Immunofluorescence analysis of CD199 was performed using CD199 Recombinant Mouse Monoclonal Antibody (BBC3M4) (Product # 740187M) on 70-80% confluent MOLT-4 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, and then blocked with 2% BSA for 1 hour at room temperature. The cells were stained with CD199 Recombinant Mouse Monoclonal Antibody (BBC3M4) (Product # 740187M, 1:100) in 0.1% BSA, in... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
740187M
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
BBC3M4
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to the chemokine receptor gene cluster region. Two alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CCR9A; CCR9B; CDw199 antigen; chemokine (C-C motif) receptor 9; G protein-coupled receptor; G protein-coupled receptor 28; unnamed protein product
Gene Aliases: CC-CKR-9; CDw199; GPR-9-6; GPR28
UniProt ID: (Human) P51686
Entrez Gene ID: (Human) 10803
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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