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TLR3/CD283 Recombinant Rabbit Monoclonal Antibody (230445G9)
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FIGURE: 1 / 3
TLR3/CD283 Antibody (98016-2-RR) in Flow
1x10^6 human monocyte-derived immature dendritic cells were intracellularly stained with 0.25 µg Anti-Human TLR3/CD283 Rabbit Recombinant Antibody (98016-2-RR, Clone: 230445G9) (red) or 0.25 µg Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG (H+L). Cells were then stained with APC Anti-Human CD11c. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Product Details
98016-2-RR
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
230445G9
Immunogen
TLR3/CD283 fusion protein Ag34044
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Wet ice
Product Specific Information
Immunogen sequence: FEGWRISFYW NVSVHRVLGF KEIDRQTEQF EYAAYIIHAY KDKDWVWEHF SSMEKEDQSL KFCLEERDFE AGVFELEAIV NSIKRSRKII FVITHHLLKD PLCKRFKVHH AVQQAIEQNL DSIILVFLEE IPDYKLNHAL CLRRGMFKSH CILNWPVQKE RIGAFRHKLQ VALGSKNSVH
Target Information
TLR3 (Toll-like receptor 3, CD283) is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from drosophila to humans and share structural and functional similarities. TLRs recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR3 is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. TLR3 recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. Further, TLR3 may play a role in host defense against viruses, and the use of alternative polyadenylation sites to generate different length transcripts has been noted for the TLR3 gene. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. TLR3 is the only human TLR which does not utilize MyD88 as an adaptor molecule instead utilizing TRIF in a signaling path which results in downstream activation of IRF-3 and NF-kB. TLR3 activation leads not only to Type I interferon induction but also to other inflammatory cytokines which result in DC maturation. TLR3 is known to recognize viral double-stranded (ds) RNA, a molecular pattern associated with viral infection. Recently it has been shown to recognize viruses such as Influenza A and West Nile Virus and can mediate entry of at least West Nile Virus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD283; TLR 3; TLR3; Toll-like receptor 3; unnamed protein product
Gene Aliases: CD283; IIAE2; IMD83; TLR3
UniProt ID: (Human) O15455
Entrez Gene ID: (Human) 7098
MAPK cascade
microglial cell activation
toll-like receptor signaling pathway
immune system process
positive regulation of immune system process
regulation of dendritic cell cytokine production
defense response
inflammatory response
hyperosmotic response
signal transduction
activation of NF-kappaB-inducing kinase activity
JNK cascade
male gonad development
detection of virus
response to virus
positive regulation of gene expression
positive regulation of type I interferon production
positive regulation of chemokine production
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of interferon-gamma production
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
toll-like receptor 3 signaling pathway
regulation of toll-like receptor 3 signaling pathway
type III interferon production
positive regulation of type III interferon production
cellular response to interferon-beta
defense response to bacterium
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
proteasome-mediated ubiquitin-dependent protein catabolic process
response to exogenous dsRNA
response to dsRNA
innate immune response
negative regulation of osteoclast differentiation
positive regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JNK cascade
positive regulation of inflammatory response
defense response to virus
positive regulation of macrophage cytokine production
cellular response to mechanical stimulus
cellular response to interferon-gamma
cellular response to exogenous dsRNA
cellular response to xenobiotic stimulus
inflammatory response to wounding
extrinsic apoptotic signaling pathway
necroptotic signaling pathway
cellular response to virus
intracellular signaling cassette
positive regulation of cytokine production involved in inflammatory response
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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