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GenScript
His Tag Monoclonal Antibody (6G2A9), Biotin, THE™
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FIGURE: 1 / 1
His Tag Antibody (A00613) in WB
Western blot analysis of His tagged fusion protein (Genscript, M0100) using THE™ His Tag Antibody [Biotin], mAb, mouse (GenScript, A00613, 1 µg/mL). The signal was developed with Streptaidin-HRP (GenScript, M00091, 1:10,000) and LumiSensor TM HRP Substrate Kit (GenScript, L00221). Predicted Size: 52 KD. Observed Size: 52 KD.
Product Details
A00613
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
6G2A9
Immunogen
A synthetic peptide (HHHHHH) coupled to KLH
Conjugate
Form
Lyophilized
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
GenScript THE™ His Tag Antibody [Biotin], mAb, mouse is the biotin conjugated THE™ His Tag Antibody, mAb, mouse (A00186) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of His in fusion proteins.
Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.
Target Information
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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