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Invitrogen
Bcl-2 Monoclonal Antibody (Bcl2/100), PE
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FIGURE: 1 / 8
Bcl-2 Antibody (A15796) in Flow
Separation of human BCL-2 positive CD19 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood from DLBCL patient stained using anti-human BCL2 (Bcl-2/100) PE Monoclonal antibody (Product # A15796) using a dilution of 10 µL reagent/100 µL of peripheral whole blood.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
A15796
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
Bcl2/100
Immunogen
Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes Bcl2, a 26 kDa intracellular protooncogen with anti-apoptotic effect, expressed in outer mitochondrial membrane, endoplasmic reticulum and nuclear envelope.
Target Information
BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl 2; bcl-2 protein; bcl2-alpha protein; bcl2-beta protein; bcl2-Ig fusion gene; OTTHUMP00000163680; protein phosphatase 1, regulatory subunit 50
Gene Aliases: Bcl-2; BCL2; PPP1R50
Entrez Gene ID: (Human) 596
protease binding
protein binding
channel activity
channel inhibitor activity
protein phosphatase binding
ubiquitin protein ligase binding
identical protein binding
sequence-specific DNA binding
protein heterodimerization activity
BH3 domain binding
protein phosphatase 2A binding
binding, bridging
DNA-binding transcription factor binding
protein polyubiquitination
release of cytochrome c from mitochondria
apoptotic process
humoral immune response
cellular response to DNA damage stimulus
female pregnancy
positive regulation of cell proliferation
extrinsic apoptotic signaling pathway via death domain receptors
intrinsic apoptotic signaling pathway in response to DNA damage
response to radiation
response to xenobiotic stimulus
response to toxic substance
response to iron ion
regulation of autophagy
negative regulation of autophagy
programmed cell death
positive regulation of cell growth
positive regulation of B cell proliferation
hair follicle morphogenesis
endoplasmic reticulum calcium ion homeostasis
response to cytokine
response to nicotine
B cell proliferation
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of apoptotic process
pigmentation
regulation of neuron apoptotic process
negative regulation of neuron apoptotic process
regulation of viral genome replication
regulation of mitochondrial membrane permeability
B cell receptor signaling pathway
neuron apoptotic process
defense response to virus
regulation of mitochondrial membrane potential
negative regulation of mitochondrial depolarization
regulation of calcium ion transport
transmembrane transport
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
extrinsic apoptotic signaling pathway in absence of ligand
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
regulation of intracellular signal transduction
negative regulation of anoikis
regulation of apoptotic signaling pathway
negative regulation of apoptotic signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
negative regulation of intrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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