Osenses

ARX Polyclonal Antibody

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ARX Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — IHC-P on paraffin sections of mouse olfactory bulb. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 mL 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

ARX Antibody in Western Blot (WB) — WB on mouse brain lysate. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1:1500 dilution incubated overnight at 4°C.

Product Details

OSA00264W-100UL

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / Ig

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from aa region 2-50 of human Homeobox protein ARX conjugated to blue carrier protein was used as the antigen

View immunogen

Conjugate

Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Product Specific Information

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

The peptide is homologous in rat and mouse.

Specificity of this antibody: ARX.

Target Information

Arx contains two conserved domains, a C-peptide (or aristaless domain) and the prd-like class homeobox domain. It is a member of the group-II aristaless-related protein family whose members are expressed primarily in the central and/or peripheral nervous system. This protein is thought to be involved in CNS development. Mutations in the Arx gene cause X-linked mental retardation and epilepsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Human ARX gene: genomic characterization and expression.

Molecular genetics and metabolism

Ohira R,Zhang YH,Guo W,Dipple K,Shih SL,Doerr J,Huang BL,Fu LJ,Abu-Khalil A,Geschwind D,McCabe ER

Tue May 27 00:00:00 EDT 2003

Mutations in the human ortholog of Aristaless cause X-linked mental retardation and epilepsy.

Nature genetics

Strømme P,Mangelsdorf ME,Shaw MA,Lower KM,Lewis SM,Bruyere H,Lütcherath V,Gedeon AK,Wallace RH,Scheffer IE,Turner G,Partington M,Frints SG,Fryns JP,Sutherland GR,Mulley JC,Gécz J

Mon Apr 01 00:00:00 EST 2002

Bioinformatics

Protein Aliases: Aristaless-related homeobox; aristaless-related homeobox, X-linked; arx {ECO:0000250|UniProtKB:Q96QS3}; cancer/testis antigen 121; Homeobox protein ARX

Gene Aliases: ARX; CT121; EIEE1; ISSX; MRX29; MRX32; MRX33; MRX36; MRX38; MRX43; MRX54; MRX76; MRX87; MRXS1; PRTS; RGD1562672

UniProt ID: (Human) Q96QS3, (Mouse) A2A4G9, (Rat) A6YP92

Entrez Gene ID: (Human) 170302, (Mouse) 11878, (Rat) 317268

Molecular Function: homeodomain transcription factor