Invitrogen

ARX Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

3 References

View all (8) ARX antibodies

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Protocols

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ARX Antibody in Western Blot (WB) — Western blot analysis of human HeLa cell lysate using an anti-ARX polyclonal antibody (Product # PA5-40407).

ARX Antibody — Figure 6. Open in a separate window Estrogen (E2) treatment elevates Arx, Shox2, and Gad65/67 levels. A , Data are shown as mean +- SEM ( n = 5 each group). mRNA expression of Arx , Shox2 , and Gad1 was higher in E2-treated kits relative to controls as indicted. GAD2 and Lhx6 were comparable between the two groups. B , Representative Western blot analyses for Arx, Lmo3, Sox6, and Dlx1 in brain homogenates from E2- and vehicle-treated kits at P3 and P7. Data are shown as mean +- SEM ( n = 5 each group). The protein levels were comparable between E2-treated kits relative to controls at both P3 and P7. C , Representative Western blot analyses for Arx, Lmo3, CXCR4, Gad65, and Gad67 in brain homogenates from E2- and vehicle-treated kits at P14. Data are shown as mean +- SEM ( n = 5 each group). Arx and GAD65 protein level was higher in E2-treated kits relative to controls at P14, whereas Lmo3 was reduced in E2-treated kits compared with controls. * p < 0.05, ** p < 0.01 for E2- versus vehicle-treated kits. {TM}

ARX Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Published figure using ARX polyclonal antibody (Product # PA5-40407) in Immunocytochemistry and Immunohistochemistry

Product Details

PA5-40407

Applications	Tested Dilution	Publications
Western Blot (WB)	0.2-1 µg/mL	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide directed towards the middle region of human ARX
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS with 2% sucrose
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2605930

Product Specific Information

Peptide sequence: AAGGGTGTED DEEELLEDEE DEDEEEELLE DDEEELLEDD ARALLKEPRR

Sequence homology: Dog: 92%; Human: 100%; Mouse: 92%; Rabbit: 100%; Rat: 92%

Target Information

Arx contains two conserved domains, a C-peptide (or aristaless domain) and the prd-like class homeobox domain. It is a member of the group-II aristaless-related protein family whose members are expressed primarily in the central and/or peripheral nervous system. This protein is thought to be involved in CNS development. Mutations in the Arx gene cause X-linked mental retardation and epilepsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Estrogen Treatment Reverses Prematurity-Induced Disruption in Cortical Interneuron Population.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Panda S,Dohare P,Jain S,Parikh N,Singla P,Mehdizadeh R,Klebe DW,Kleinman GM,Cheng B,Ballabh P

Published figure using ARX polyclonal antibody (Product # PA5-40407) in Immunofluorescence

Wed Aug 22 00:00:00 EDT 2018

Estrogen Treatment Reverses Prematurity-Induced Disruption in Cortical Interneuron Population.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Panda S,Dohare P,Jain S,Parikh N,Singla P,Mehdizadeh R,Klebe DW,Kleinman GM,Cheng B,Ballabh P

Published figure using ARX polyclonal antibody (Product # PA5-40407) in Immunofluorescence

Wed Aug 22 00:00:00 EDT 2018

Estrogen Treatment Reverses Prematurity-Induced Disruption in Cortical Interneuron Population.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Panda S,Dohare P,Jain S,Parikh N,Singla P,Mehdizadeh R,Klebe DW,Kleinman GM,Cheng B,Ballabh P

Published figure using ARX polyclonal antibody (Product # PA5-40407) in Immunofluorescence

Wed Aug 22 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Aristaless-related homeobox; aristaless-related homeobox, X-linked; cancer/testis antigen 121; Homeobox protein ARX

Gene Aliases: ARX; CT121; EIEE1; ISSX; MRX29; MRX32; MRX33; MRX36; MRX38; MRX43; MRX54; MRX76; MRX87; MRXS1; PRTS

UniProt ID: (Human) Q96QS3

Entrez Gene ID: (Human) 170302

Molecular Function: homeodomain transcription factor

Function(s)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated axon guidance positive regulation of gene expression globus pallidus development cerebral cortex tangential migration embryonic olfactory bulb interneuron precursor migration cell proliferation in forebrain cerebral cortex GABAergic interneuron migration regulation of cell proliferation lipid digestion positive regulation of organ growth epithelial cell fate commitment

Process(es)

RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II distal enhancer sequence-specific binding chromatin binding

ARX Polyclonal Antibody

Product Details

PA5-40407

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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