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Invitrogen
ATG12 Polyclonal Antibody
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Product Details
PA5-32180
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ATG12. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.43 mg/mL
Amount
143 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, Rat brain, ATG12 shRNA-transfected 293T.
Predicted reactivity: Pig (81%), Chimpanzee (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ATG12, a member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist. Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: APG 12; Apg12 (autophagy, yeast) homolog; APG12 autophagy 12-like; APG12-like; ATG12 autophagy related 12 homolog; autophagy-related 12; Autophagy-related protein 12; OTTHUMP00000159129; ubiquitin-like protein; Ubiquitin-like protein ATG12; unnamed protein product
Gene Aliases: 4931423H11Rik; A330058M13Rik; APG12; APG12L; ATG12; Atg12l; FBR93; HAPG12
UniProt ID: (Human) O94817, (Rat) Q2TBJ5, (Mouse) Q9CQY1
Entrez Gene ID: (Human) 9140, (Rat) 361321, (Mouse) 67526
autophagosome assembly
mitophagy
autophagy
macroautophagy
negative regulation of type I interferon production
piecemeal microautophagy of nucleus
negative regulation of innate immune response
negative regulation of defense response to virus
glycophagy
autophagosome maturation
regulation of autophagosome maturation
positive regulation of viral translation
C-terminal protein lipidation
cellular response to nitrogen starvation
nucleophagy
innate immune response
immune system process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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