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ATG5 Polyclonal Antibody

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ATG5 Antibody in Immunocytochemistry (ICC/IF) — IF result of anti-ATG5 (10181-2-AP) with E15 mouse cortical neurons.

ATG5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10181-2-AP ( ATG5 antibody) at dilution of 1:200 (under 40x lens).

ATG5 Antibody in Western Blot (WB) — Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

10181-2-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 41 publications 41 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:20-1:200	View 2 publications 2 publications
Flow Cytometry (Flow)	Assay-dependent	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Artificial Control, Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ATG5 Fusion Protein Ag0214 (28-275 aa encoded by BC002699) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.27 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band. 10181-2-AP also recognizes the free ATG5 (32 kDa).

Immunogen sequence: TEREAEPYY LLLPRVSYLT LVTDKVKKHF QKVMRQEDIS EIWFEYEGTP LKWHYPIGLL FDLLASSSAL PWNITVHFKS FPEKDLLHCP SKDAIEAHFM SCMKEADALK HKSQVINEMQ KKDHKQLWMG LQNDRFDQFW AINRKLMEYP AEENGFRYIP FRIYQTTTER PFIQKLFRPV AADGQLHTLG DLLKEVCPSA IDPEDGEKKN QVMIHGIEPM LETPLQWLSE HLSYPDNFLH ISIIPQPTD (28-275 aa encoded by BC002699)

Target Information

ATG5 (Autophagy Related 5) is an important element for autophagy and may play an important role in the apoptotic process. ATG5 is also involved in other cellular processes that include mitochondrial quality control after oxidative damage, negative regulation of the innate anti-viral immune response, lymphocyte development and proliferation, MHC II antigen presentation, and adipocyte differentiation. Following conjugation to ATG12, the conjugate participates in the formation of autophagosome. ATG5 contributes to autophagic cell death by interacting with Fas-associated protein with death domain (FADD). The ATG5-ATG12 conjugate forms a cup-shaped isolation membrane that then detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may play a role in the apoptotic process, possibly within the modified cytoskeleton. Further, APG5 expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. The APG5-APG12 conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. Diseases associated with ATG5 dysfunction include spinocerebellar ataxia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: APG 5; APG5 autophagy 5 like (APG5L); APG5-like; Apoptosis-specific protein; Apoptosis-specific protein (ASP); ATG5 autophagy related 5 homolog; Autophagy protein 5; autophagy-related 5

Gene Aliases: 2010107M05Rik; 3110067M24Rik; APG5; APG5-LIKE; APG5L; ASP; ATG5; Atg5l; AW319544; C88337; hAPG5; Paddy

UniProt ID: (Human) Q9H1Y0, (Mouse) Q99J83

Entrez Gene ID: (Human) 9474, (Mouse) 11793, (Rat) 365601

Molecular Function: membrane trafficking regulatory protein