|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 383 and 553 of Human ATP5A1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
PA5-27504 targets ATP5A1 in Western blot and immunohistochemistry (paraffin) applications and shows reactivity with Human and mouse samples.
The PA5-27504 immunogen is recombinant fragment corresponding to a region within amino acids 383 and 553 of Human ATP5A1.
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the same protein have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Nuclear cytoplasmic trafficking of proteins is a major response of human fibroblasts to oxidative stress.
PA5-27504 was used in immunocytochemistry to measure nuclear and cytoplasmic protein abundances in human IMR90 fibroblasts subjected to mild oxidative stress using a subcellular spatial razor approach
|Baqader NO,Radulovic M,Crawford M,Stoeber K,Godovac-Zimmermann J||Journal of proteome research (13:4398)||2014|