Invitrogen

Annexin V Monoclonal Antibody (VAA-33), FITC, eBioscience™

Product Details

BMS147FI

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	Assay-Dependent	View 18 publications 18 publications

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse, Rat
Host/Isotype	Mouse / IgG2a, kappa
Recommended Isotype Control	Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	VAA-33
Conjugate	FITC Biotin Unconjugated
Excitation/Emission Max	498/517 nm View spectra
Form	Liquid
Concentration	10 µL/Test
Purification	Affinity chromatography
Storage buffer	TBS, pH 7.2-7.4, with 1% BSA
Contains	0.02% sodium azide
Storage conditions	4° C, store in dark
RRID	AB_10597608

Product Specific Information

Description: BMS147 specifically recognizes Annexin V (VAC- alpha ). BMS147 is suitable for ELISA application, Western blot analysis and is suitable as primary antibody for staining in FACS analysis.

BMS147FI can be used for direct staining of cells.

BMS147BT can be used in a two-step procedure using the biotin-(strept-)avidin system. For intracellular stainings the use of BMS147FI is recommended.

Applications Tested: Flow Cytometry (Intracellular Staining).

Purity: >95%.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser

Target Information

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

Panel Builder

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.

Oncoimmunology

Catakovic K,Gassner FJ,Ratswohl C,Zaborsky N,Rebhandl S,Schubert M,Steiner M,Gutjahr JC,Pleyer L,Egle A,Hartmann TN,Greil R,Geisberger R

BMS147FI was used in Flow cytometry/Cell sorting to reveal that TIGIT+CD4+T cells provide a supportive microenvironment for CLL cells.

Sat Jan 29 00:00:00 EST 2022

Sleep enhances numbers and function of monocytes and improves bacterial infection outcome in mice.

Brain, behavior, and immunity

Hahn J,Günter M,Schuhmacher J,Bieber K,Pöschel S,Schütz M,Engelhardt B,Oster H,Sina C,Lange T,Autenrieth SE

BMS147FI was used in Flow Cytometry to elucidate whether sleep affects the numbers and functions of innate immune cells and their defense against systemic bacterial infection.

Wed Jul 01 00:00:00 EDT 2020

N6-isopentenyladenosine dual targeting of AMPK and Rab7 prenylation inhibits melanoma growth through the impairment of autophagic flux.

Cell death and differentiation

Ranieri R,Ciaglia E,Amodio G,Picardi P,Proto MC,Gazzerro P,Laezza C,Remondelli P,Bifulco M,Pisanti S

BMS147FI was used in Flow cytometry/Cell sorting to identify N6-isopentenyladenosine, an end product of the mevalonate pathway, as a novel autophagy inhibitor with an interesting anti-melanoma activity.

Thu Feb 01 00:00:00 EST 2018

Interleukin-1 receptor-associated kinase 3 downregulation in peripheral blood mononuclear cells attenuates immunosuppression in sepsis.

Experimental and therapeutic medicine

Xia Q,Zhou Y,Wang X,Fu S

BMS147FI was used in Flow cytometry/Cell sorting to evaluate the expression and function of IL-1 receptor-associated kinase 3 in blood mononuclear cells from a model of sepsis-induced immunosuppression.

Thu Feb 01 00:00:00 EST 2018

Selective blockade of CD28 on human T cells facilitates regulation of alloimmune responses.

JCI insight

Zaitsu M,Issa F,Hester J,Vanhove B,Wood KJ

BMS147FI was used in Flow cytometry/Cell sorting to show that a nonactivating monovalent anti-CD28 that spares CTLA4 signaling is an effective immunosuppressant in a clinically relevant humanized mouse transplant model.

Thu Oct 05 00:00:00 EDT 2017

Blocking downstream signaling pathways in the context of HDAC inhibition promotes apoptosis preferentially in cells harboring mutant Ras.

Oncotarget

Bahr JC,Robey RW,Luchenko V,Basseville A,Chakraborty AR,Kozlowski H,Pauly GT,Patel P,Schneider JP,Gottesman MM,Bates SE

BMS147FI was used in Flow cytometry/Cell sorting to investigate whether combining romidepsin with small molecule pathway inhibitors would lead to increased apoptosis in cancers harboring Ras mutations.

Tue Oct 25 00:00:00 EDT 2016

Fluorescent magnetic iron oxide nanoparticles for cardiac precursor cell selection from stromal vascular fraction and optimization for magnetic resonance imaging.

International journal of nanomedicine

Verma VK,Kamaraju SR,Kancherla R,Kona LK,Beevi SS,Debnath T,Usha SP,Vadapalli R,Arbab AS,Chelluri LK

BMS147FI was used in Flow cytometry/Cell sorting to develop a nanoprobe for cell selection and imaging with a direct therapeutic translational focus.

Tue Aug 16 00:00:00 EDT 2016

Mitochondrial Damage-Associated Molecular Patterns (MTDs) Are Released during Hepatic Ischemia Reperfusion and Induce Inflammatory Responses.

PloS one

Hu Q,Wood CR,Cimen S,Venkatachalam AB,Alwayn IP

BMS147FI was used in Flow cytometry/Cell sorting to investigate the role of mitochondrial damage-associate molecular patterns in the pathogenesis of hepatic ischemia/reperfusion injury.

Wed Jul 06 00:00:00 EDT 2016

Mitochondrial Damage-Associated Molecular Patterns (MTDs) Are Released during Hepatic Ischemia Reperfusion and Induce Inflammatory Responses.

PloS one

Hu Q,Wood CR,Cimen S,Venkatachalam AB,Alwayn IP

BMS147FI was used in Flow cytometry/Cell sorting to investigate the role of mitochondrial damage-associate molecular patterns in the pathogenesis of hepatic ischemia/reperfusion injury.

Wed Jul 06 00:00:00 EDT 2016

Sulforaphane Protects the Liver against CdSe Quantum Dot-Induced Cytotoxicity.

PloS one

Wang W,He Y,Yu G,Li B,Sexton DW,Wileman T,Roberts AA,Hamilton CJ,Liu R,Chao Y,Shan Y,Bao Y

BMS147FI was used in Flow cytometry/Cell sorting to assess the ability of sulforaphane to attenuate the cytotoxic side-effects of a diagnostic agent.

Tue Jun 07 00:00:00 EDT 2016

A single exercise bout enhances the manufacture of viral-specific T-cells from healthy donors: implications for allogeneic adoptive transfer immunotherapy.

Scientific reports

Spielmann G,Bollard CM,Kunz H,Hanley PJ,Simpson RJ

BMS147FI was used in Flow cytometry/Cell sorting to demonstrate that a single exercise bout enhances the manufacture of multi-VSTs from healthy donors without altering their phenotype or function.

Mon May 16 00:00:00 EDT 2016

Suppression of established hepatocarcinoma in adjuvant only immunotherapy: alum triggers anti-tumor CD8+ T cell response.

Scientific reports

Wang B,Wang X,Wen Y,Fu J,Wang H,Ma Z,Shi Y,Wang B

BMS147FI was used in Flow cytometry/Cell sorting to investigate the use of alum in adjuvant immunotherapy.

Wed Dec 09 00:00:00 EST 2015

Overexpression of CX3CR1 is associated with cellular metastasis, proliferation and survival in gastric cancer.

Oncology reports

Wei LM,Cao S,Yu WD,Liu YL,Wang JT

BMS147FI was used in Flow cytometry/Cell sorting to investigate whether CX3CR1 is expressed in gastric cancer cells and whether it participates in gastric cancer metastasis.

Sun Feb 01 00:00:00 EST 2015

Fas/Fas ligand mediates keratinocyte death in sunitinib-induced hand-foot skin reaction.

The Journal of investigative dermatology

Yeh CN,Chung WH,Su SC,Chen YY,Cheng CT,Lin YL,Chang WC,Chung-Yee Hui R,Chiang KC,Chen TW,Jan YY,Chen CW,Chen TJ,Yang CH,Hung SI

BMS147FI was used in Immunohistochemistry to assess differences in protein expression between gastrointestinal stromal cancer sufferers who experience hand-foot skin reaction to those who do not.

Sat Nov 01 00:00:00 EDT 2014

Induction of cell cycle arrest and apoptosis in caspase-3 deficient MCF-7 cells by Dillenia suffruticosa root extract via multiple signalling pathways.

BMC complementary and alternative medicine

Foo JB,Yazan LS,Tor YS,Armania N,Ismail N,Imam MU,Yeap SK,Cheah YK,Abdullah R,Ismail M

BMS147FI was used in Flow cytometry/Cell sorting to assess the potential for Dillenia suffruticosa root extract to be used to induce apoptosis in caspase-3 deficient cancer cells.

Thu Jun 19 00:00:00 EDT 2014

Id1 expression promotes peripheral CD4+ T cell proliferation and survival upon TCR activation without co-stimulation.

Biochemical and biophysical research communications

Liu C,Jin R,Wang HC,Tang H,Liu YF,Qian XP,Sun XY,Ge Q,Sun XH,Zhang Y

BMS147FI was used in Flow cytometry/Cell sorting to study the role of Id1 in the regulation of E proteins and activation and proliferation of T cells.

Fri Jun 21 00:00:00 EDT 2013

Alteration of CXCR7 expression mediated by TLR4 promotes tumor cell proliferation and migration in human colorectal carcinoma.

PloS one

Xu H,Wu Q,Dang S,Jin M,Xu J,Cheng Y,Pan M,Wu Y,Zhang C,Zhang Y

BMS147FI was used in Flow cytometry/Cell sorting to investigate the impact of LPS binding to TLR4 on chemokine receptors in human colorectal carcinoma cells.

Wed Apr 11 00:00:00 EDT 2012

The impact of inflammatory licensing on heme oxygenase-1-mediated induction of regulatory T cells by human mesenchymal stem cells.

Blood

Mougiakakos D,Jitschin R,Johansson CC,Okita R,Kiessling R,Le Blanc K

BMS147FI was used in Flow cytometry/Cell sorting to demonstrate how HO-1 produced by human MSCs beyond its direct suppressive function promotes formation of Tr1 and Th3 Tregs and IL-10 production.

Thu May 05 00:00:00 EDT 2011

Increased thioredoxin-1 production in human naturally occurring regulatory T cells confers enhanced tolerance to oxidative stress.

Blood

Mougiakakos D,Johansson CC,Jitschin R,Böttcher M,Kiessling R

BMS147FI was used in Flow cytometry/Cell sorting to demonstrate that human Tregs express and secrete higher levels of thioredoxin-1, a major antioxidative molecule.

Thu Jan 20 00:00:00 EST 2011

Presence of Foxp3-expressing CD19(+)CD5(+) B Cells in Human Peripheral Blood Mononuclear Cells: Human CD19(+)CD5(+)Foxp3(+) Regulatory B Cell (Breg).

Immune network

Noh J,Choi WS,Noh G,Lee JH

BMS147FI was used in Flow cytometry/Cell sorting to investigate the role of Foxp3 in B cells.

Wed Dec 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: Anchorin CII; Annexin A5; Annexin V; Annexin-5; Calphobindin I; CBP-I; CPB-I; Endonexin II; epididymis secretory protein Li 7; Lipocortin V; PAP-I; Placental anticoagulant protein 4; Placental anticoagulant protein I; PP4; Thromboplastin inhibitor; VAC-alpha; Vascular anticoagulant-alpha

Gene Aliases: ANX5; ANXA5; ENX2; HEL-S-7; PP4; RPRGL3

UniProt ID: (Human) P08758

Entrez Gene ID: (Human) 308

Molecular Function: calcium-binding protein

Function(s)

positive regulation of defense response to virus by host signal transduction blood coagulation negative regulation of blood coagulation positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of catalytic activity protein homooligomerization response to calcium ion calcium ion transmembrane transport cellular response to lead ion response to thyroid hormone cellular response to gonadotropin-releasing hormone mitophagy in response to mitochondrial depolarization xenophagy regulation of sperm motility negative regulation of prolactin secretion

Process(es)

phospholipase inhibitor activity calcium-transporting ATPase activity calcium ion binding protein binding phospholipid binding calcium-dependent phospholipid binding peptide hormone binding receptor tyrosine kinase binding

Annexin V Monoclonal Antibody (VAA-33), FITC, eBioscience™

Product Details

BMS147FI

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!