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Axl Recombinant Rat Monoclonal Antibody (MAXL8DS), Alexa Fluor™ Plus 488
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FIGURE: 1 / 3
Axl Antibody (750031TP488) in Flow
Flow cytometry analysis of Axl Recombinant Rat Monoclonal Antibody (MAXL8DS), Alexa Fluor™ Plus 488 (Product # 750031TP488) was performed using NIH/3T3 and Neuro-2a cells. Cells were fixed with eBioscience™ IC Fixation Buffer (Product # 00-8222-49 and stained with 0.25 µg of the of Axl Recombinant Rat Monoclonal Antibody (MAXL8DS), Alexa Fluor™ Plus 488 (Product # 750031TP488). Higher expression of Axl was observed in NIH/3T3 (pink histogram) in comparison to Neuro-2a (blue histogram). Viable cells were used for analysis as determined by L... View More
Product Details
750031TP488
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
MAXL8DS
Conjugate
Excitation/Emission Max
493/518 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
proprietary buffer, pH 6.3-7.3
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma. The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adhesion-related kinase; AZF1; oncogene AXL; sAXL; soluble AXL; Tyrosine-protein kinase receptor UFO; ufo oncogene homolog
Gene Aliases: AI323647; Ark; Axl; Tyro7; Ufo
UniProt ID: (Mouse) Q00993
Entrez Gene ID: (Mouse) 26362
nucleotide binding
phosphatidylserine binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
myosin heavy chain binding
phosphatidylinositol 3-kinase binding
protein heterodimerization activity
transmembrane signal receptor
neuron migration
natural killer cell differentiation
positive regulation of cytokine-mediated signaling pathway
blood vessel remodeling
immune system process
protein phosphorylation
phagocytosis
inflammatory response
enzyme linked receptor protein signaling pathway
spermatogenesis
phosphorylation
peptidyl-tyrosine phosphorylation
forebrain cell migration
cell differentiation
platelet activation
cellular response to extracellular stimulus
negative regulation of interferon-gamma production
negative regulation of tumor necrosis factor production
positive regulation of natural killer cell differentiation
secretion by cell
erythrocyte homeostasis
substrate adhesion-dependent cell spreading
cellular response to interferon-alpha
ovulation cycle
negative regulation of apoptotic process
apoptotic cell clearance
protein kinase B signaling
negative regulation of neuron apoptotic process
innate immune response
viral entry into host cell
positive regulation of pinocytosis
negative regulation of lymphocyte activation
positive regulation of protein kinase B signaling
vagina development
cellular response to hydrogen peroxide
cellular response to lipopolysaccharide
negative regulation of dendritic cell apoptotic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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