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          • Primary Antibodies ›
          • BCL-2 Antibodies

          Bioss

          Bcl-2 Polyclonal Antibody

          View all (197) BCL-2 antibodies

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          Cite Bcl-2 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (6)
          Bcl-2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Bcl-2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          Bcl-2 Antibody (BS-0032R) in ICC/IF

          Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bcl-2) polyclonal Antibody, Unconjugated (bs-0032R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Bcl-2 Antibody in Immunocytochemistry (ICC/IF)
          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Bcl-2 Antibody in Western Blot (WB)
          Bcl-2 Antibody in Western Blot (WB)
          Bcl-2 Polyclonal Antibody

          Product Details

          BS-0032R

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:300-1:5,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          Assay-dependent
          -

          Immunohistochemistry (Frozen) (IHC (F))

          1:100-500
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          -

          Flow Cytometry (Flow)

          1:20-1:100
          -

          ELISA (ELISA)

          1:500-1:1,000
          -
          Product Specifications

          Species Reactivity

          Bacteria, Bovine, Chicken, Human, Mouse, Rabbit, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          KLH conjugated synthetic peptide derived from rat Bcl-2
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          TBS, pH 7.4, with 50% glycerol, 1% BSA

          Contains

          0.03% ProClin 300

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Target Information

          BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Apoptosis regulator Bcl-2; B cell lymphoma 2 associated oncogene; B-cell CLL/lymphoma 2; B-cell leukemia/lymphoma 2; Bcl 2; bcl-2 protein; bcl2-alpha protein; bcl2-beta protein; bcl2-Ig fusion gene; Bcl2-like protein; OTTHUMP00000163680; protein phosphatase 1, regulatory subunit 50

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          Gene Aliases: AW986256; BCL-2; BCL2; C430015F12Rik; D630044D05Rik; D830018M01Rik; PCKBCL2; PPP1R50

          View more View less

          UniProt ID: (Chicken) Q00709, (Human) P10415, (Rat) P49950, (Mouse) P10417

          View more View less

          Entrez Gene ID: (Chicken) 396282, (Human) 596, (Rabbit) 103349397, (Bovine) 281020, (Rat) 24224, (Mouse) 12043

          View more View less

          Function(s)
          protease binding channel activity channel inhibitor activity ubiquitin protein ligase binding protein homodimerization activity sequence-specific DNA binding protein heterodimerization activity BH3 domain binding protein phosphatase 2A binding repressing transcription factor binding protein binding protein phosphatase binding identical protein binding binding, bridging DNA-binding transcription factor binding transcription factor binding BH domain binding
          Process(es)
          protein polyubiquitination ossification ovarian follicle development metanephros development branching involved in ureteric bud morphogenesis behavioral fear response B cell homeostasis release of cytochrome c from mitochondria regulation of cell-matrix adhesion lymphoid progenitor cell differentiation B cell lineage commitment response to ischemia renal system process protein dephosphorylation melanin metabolic process regulation of nitrogen utilization actin filament organization axonogenesis cell aging male gonad development extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage intrinsic apoptotic signaling pathway in response to oxidative stress response to toxic substance post-embryonic development response to iron ion response to UV-B response to gamma radiation regulation of gene expression negative regulation of autophagy negative regulation of calcium ion transport into cytosol regulation of glycoprotein biosynthetic process mesenchymal cell development positive regulation of neuron maturation positive regulation of smooth muscle cell migration peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cochlear nucleus development gland morphogenesis regulation of transmembrane transporter activity negative regulation of ossification positive regulation of cell growth negative regulation of cell growth melanocyte differentiation negative regulation of cell migration positive regulation of B cell proliferation axon regeneration regulation of protein stability endoplasmic reticulum calcium ion homeostasis glomerulus development negative regulation of cellular pH reduction regulation of protein localization negative regulation of myeloid cell apoptotic process T cell differentiation in thymus positive regulation of peptidyl-serine phosphorylation negative regulation of osteoblast proliferation response to cytokine response to nicotine organ growth positive regulation of multicellular organism growth B cell proliferation cellular response to glucose starvation response to drug response to hydrogen peroxide T cell homeostasis negative regulation of apoptotic process positive regulation of catalytic activity CD8-positive, alpha-beta T cell lineage commitment negative regulation of neuron apoptotic process ear development regulation of viral genome replication positive regulation of melanocyte differentiation negative regulation of retinal cell programmed cell death regulation of mitochondrial membrane permeability focal adhesion assembly spleen development thymus development digestive tract morphogenesis oocyte development positive regulation of skeletal muscle fiber development pigment granule organization homeostasis of number of cells within a tissue B cell receptor signaling pathway response to glucocorticoid neuron apoptotic process defense response to virus regulation of mitochondrial membrane potential transmembrane transport intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress lens fiber cell differentiation cellular response to organic substance cellular response to hypoxia reactive oxygen species metabolic process extrinsic apoptotic signaling pathway in absence of ligand cell-cell adhesion negative regulation of G1/S transition of mitotic cell cycle negative regulation of reactive oxygen species metabolic process negative regulation of anoikis negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway G1/S transition of mitotic cell cycle mitotic cell cycle cell morphogenesis ureteric bud development leukocyte homeostasis B cell apoptotic process kidney development lymphocyte homeostasis T cell lineage commitment immune system development negative regulation of B cell apoptotic process cellular calcium ion homeostasis autophagy apoptotic process humoral immune response cellular response to DNA damage stimulus response to oxidative stress female pregnancy cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation apoptotic mitochondrial changes response to radiation response to xenobiotic stimulus organ morphogenesis regulation of autophagy smooth muscle cell migration cell migration hemopoiesis B cell differentiation T cell differentiation hair follicle morphogenesis myeloid cell apoptotic process osteoblast proliferation regulation of growth neuron maturation regulation of apoptotic process positive regulation of apoptotic process regulation of programmed cell death pigmentation negative regulation of mitotic cell cycle retinal cell programmed cell death developmental pigmentation regulation of developmental pigmentation positive regulation of developmental pigmentation response to steroid hormone developmental growth skeletal muscle fiber development stem cell development establishment of localization in cell regulation of cell cycle negative regulation of mitochondrial depolarization regulation of calcium ion transport hematopoietic stem cell differentiation calcium ion transport into cytosol lymphocyte apoptotic process negative regulation of lymphocyte apoptotic process T cell apoptotic process negative regulation of T cell apoptotic process dendritic cell apoptotic process motor neuron apoptotic process negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator regulation of intracellular signal transduction epithelial cell apoptotic process negative regulation of epithelial cell apoptotic process negative regulation of dendritic cell apoptotic process negative regulation of motor neuron apoptotic process regulation of apoptotic signaling pathway negative regulation of apoptotic signaling pathway response to hypoxia brain development aging response to nutrient response to heat response to organic substance response to inorganic substance response to aluminum ion response to organic cyclic compound cell growth single organismal cell-cell adhesion response to caffeine response to insulin response to L-ascorbic acid glial cell apoptotic process growth response to peptide hormone regulation of protein homodimerization activity regulation of protein heterodimerization activity response to estrogen response to ethanol response to copper ion oligodendrocyte differentiation regulation of catalytic activity response to corticosterone response to cAMP response to folic acid response to electrical stimulus response to acid chemical
          It has to be done as per old AB suggested Products section.

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