A suggested positive control is Daudi cell lysate.
PA5-20068 can be used with blocking peptide PEP-0187.
The PA5-20068 immunogen is located within the first 50 amino acids of Bcl-2.
Predicted molecular ~ 26kD. In Western blot applications, this antibody has been observed to detect a band at: 26 kD.
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rat: (100%), Chicken: (93%)
BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.
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Protein Aliases: Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; B-cell leukemia/lymphoma 2; Bcl-2, PPP1R50; protein phosphatase 1, regulatory subunit 50
Gene Aliases: AW986256; Bcl-2; BCL2; C430015F12Rik; D630044D05Rik; D830018M01Rik; PPP1R50
Molecular Function: signaling molecule