|Flow Cytometry (Flow)||5 µL (0.5 µg)/test|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, store in dark, DO NOT FREEZE!|
Description: The eBioJM7A4 monoclonal antibody reacts with the IL-15 receptor alpha chain (IL-15Ralpha). IL-15 and IL-2 are cytokines with overlapping, but distinct, biologic effects. Their receptors share 2 subunits, the IL-2Rbeta and gamma chains, which are essential for signal transduction. The IL-15Ralpha receptor is structurally related to IL-2Ralpha. However, the IL-15Ralpha alone binds IL-15 with a 1,000-fold higher affinity than that seen with IL-2Ralpha and IL-2. In the human, 3 differentially spliced human IL-15Ralpha variants that are all capable of high affinity binding of IL-15 have been isolated. The cytoplasmic domain of IL-15Ralpha, like that of IL-2Ralpha, is dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high affinity binding. At high concentrations, IL-15, like IL-2, is able to signal through a complex of IL-2Rbeta and IL-2Rgamma in the absence of the alpha subunit.
Applications Reported: This eBioJM7A4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioJM7A4 antibody has been pre-titrated and tested by flow cytometric analysis of LPS and IFN gamma-stimulated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
Interleukin 15 (IL-15RA) is a cytokine receptor that specifically binds IL-15 with high affinity. IL-15 regulates T and natural killer cell activation and proliferation. The IL-15 and IL-2 receptors share two subunits, IL-2R beta and IL-2R gamma, and IL-15RA is structurally related to IL-2RA, an additional IL-2-specific alpha subunit necessary for high affinity IL-2 binding. Unlike IL-2RA, IL-15RA is capable of binding IL-15 with high affinity independent of the other subunits. IL-15RA is thought to enhance cell proliferation and expression of apoptosis inhibitor Bcl-xL and Bcl-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD215; IL-15 receptor subunit alpha; IL-15RA; IL15RA; interleukin 15 receptor alpha isoform EM2; interleukin 15 receptor alpha isoform IC2; interleukin 15 receptor alpha isoform IC3; interleukin 15 receptor alpha isoform IC4; interleukin 15 receptor alpha isoform IC5; interleukin 15 receptor alpha isoform IC6; interleukin 15 receptor alpha isoform IC7; interleukin 15 receptor alpha isoform IC8; interleukin 15 receptor, alpha; Interleukin-15 receptor subunit alpha
Gene Aliases: CD215; IL15RA
UniProt ID: (Human) Q13261
Entrez Gene ID: (Human) 3601
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