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CD215 (IL-15Ra) Monoclonal Antibody (eBioJM7A4), PE-Cyanine7, eBioscience™
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FIGURE: 1 / 1
CD215 (IL-15Ra) Antibody (25-7159-42) in Flow
Normal human peripheral blood cells were stimulated overnight with LPS (Product # 00-4976-03) and then stained with CD11b Monoclonal Antibody, APC (Product # 17-0118-42) and Mouse IgG2b kappa Isotype Control, PE-Cyanine7 (Product # 25-4732-81) (left) or CD215 Monoclonal Antibody, PE-Cyanine7 (right). Viable cells in the monocyte gate were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
25-7159-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBioJM7A4
Conjugate
PE-Cyanine7
PE-Cyanine7
PE-Cyanine7
Excitation/Emission Max
569/780 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBioJM7A4 monoclonal antibody reacts with the IL-15 receptor alpha chain (IL-15R alpha). IL-15 and IL-2 are cytokines with overlapping, but distinct, biologic effects. Their receptors share 2 subunits, the IL-2Rbeta and gamma chains, which are essential for signal transduction. The IL-15Ralpha receptor is structurally related to IL-2Ralpha. However, the IL-15Ralpha alone binds IL-15 with a 1,000-fold higher affinity than that seen with IL-2Ralpha and IL-2. In the human, 3 differentially spliced human IL-15Ralpha variants that are all capable of high affinity binding of IL-15 have been isolated. The cytoplasmic domain of IL-15Ralpha, like that of IL-2Ralpha, is dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high affinity binding. At high concentrations, IL-15, like IL-2, is able to signal through a complex of IL-2Rbeta and IL-2Rgamma in the absence of the alpha subunit.
Applications Reported: This eBioJM7A4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioJM7A4 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Target Information
Interleukin 15 (IL-15RA) is a cytokine receptor that specifically binds IL-15 with high affinity. IL-15 regulates T and natural killer cell activation and proliferation. The IL-15 and IL-2 receptors share two subunits, IL-2R beta and IL-2R gamma, and IL-15RA is structurally related to IL-2RA, an additional IL-2-specific alpha subunit necessary for high affinity IL-2 binding. Unlike IL-2RA, IL-15RA is capable of binding IL-15 with high affinity independent of the other subunits. IL-15RA is thought to enhance cell proliferation and expression of apoptosis inhibitor Bcl-xL and Bcl-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytokine; IL 15 receptor; IL 15 receptor subunit α; IL 15R; IL 15R alpha; IL 15R α; IL15 receptor; IL15 receptor subunit alpha; IL15 receptor subunit α; IL15R; IL15R alpha; IL15R α; IL15RA; Interleukin 15 receptor; Interleukin 15 receptor subunit α; interleukin 15 receptor, alpha; sIL 15R; sIL-15R-alpha; sIL-15RA; sIL15R; similar to interleukin-15 receptor alpha chain precursor; soluble IL 15R; soluble IL15R; unnamed protein product
Gene Aliases: CD215
UniProt ID: (Human) Q13261
Entrez Gene ID: (Human) 3601
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