Bioss

CD28 Polyclonal Antibody

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers
Tech Support
CD28 Polyclonal Antibody

Product Details

BS-1297R

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:400
-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

Flow Cytometry (Flow)

1:100
-

ELISA (ELISA)

1:500-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from mouse CD28, amino acids 1-120.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD28; CD28 antigen; MGC138290; sCD28; soluble CD28; T-cell-specific surface glycoprotein CD28; T44; TP44

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Gene Aliases: CD28; CD28RNA; Tp44

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UniProt ID: (Human) P10747, (Mouse) P31041, (Rat) P31042

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Entrez Gene ID: (Human) 940, (Mouse) 12487, (Rat) 25660

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Function(s)
protease binding SH3/SH2 adaptor activity protein binding coreceptor activity protein kinase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein homodimerization activity immunoglobulin receptor superfamily
Process(es)
positive regulation of inflammatory response to antigenic stimulus humoral immune response cell surface receptor signaling pathway positive regulation of gene expression negative regulation of gene expression regulation of phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling T cell costimulation positive regulation of interleukin-10 production positive regulation of interleukin-4 production cytokine biosynthetic process positive regulation of T cell proliferation negative thymic T cell selection regulatory T cell differentiation positive regulation of viral genome replication positive regulation of interleukin-2 biosynthetic process regulation of regulatory T cell differentiation positive regulation of translation positive regulation of mitotic nuclear division positive regulation of transcription from RNA polymerase II promoter positive regulation of alpha-beta T cell proliferation phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling positive regulation of isotype switching to IgG isotypes regulation of defense response to virus by virus T cell receptor signaling pathway positive regulation of protein kinase B signaling apoptotic signaling pathway immune response positive regulation of signal transduction T cell activation signal transduction
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