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|Tested species reactivity||Human|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG2a|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD4 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD4 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD4 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
Analyte Specific Reagent
IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia.
MHCD0400 was used in flow cytometry to developed a strategy to expand umbilical cord blood T cells and test their effects after transplantation
|Pegram HJ,Purdon TJ,van Leeuwen DG,Curran KJ,Giralt SA,Barker JN,Brentjens RJ||Leukemia (29:415)||2015|
Nef neutralizes the ability of exosomes from CD4+ T cells to act as decoys during HIV-1 infection.
MHCD0400 was used in flow cytometry and immunocytochemistry to test if Nef modifies the composition of exosomes released by T lymphocytes
|de Carvalho JV,de Castro RO,da Silva EZ,Silveira PP,da Silva-Januário ME,Arruda E,Jamur MC,Oliver C,Aguiar RS,daSilva LL||PloS one (9:null)||2014|
Polarized axonal surface expression of neuronal KCNQ potassium channels is regulated by calmodulin interaction with KCNQ2 subunit.
MHCD0400 was used in immunocytochemistry to identify the molecular mechanisms that regulate the enrichment of KCNQ channels at the neuronal axon
|Cavaretta JP,Sherer KR,Lee KY,Kim EH,Issema RS,Chung HJ||PloS one (9:null)||2014|
Reduced cell surface expression of CCR5 in CCR5Delta 32 heterozygotes is mediated by gene dosage, rather than by receptor sequestration.
MHCD0400 was used in flow cytometry to determine how CCR5 expression is reduced in cells from ccr5Delta32 heterozygotes.
|Venkatesan S,Petrovic A,Van Ryk DI,Locati M,Weissman D,Murphy PM||The Journal of biological chemistry (277:2287)||2002|
A membrane-proximal basic domain and cysteine cluster in the C-terminal tail of CCR5 constitute a bipartite motif critical for cell surface expression.
MHCD0400 was used in flow cytometry to characterize CCR5 truncations.
|Venkatesan S,Petrovic A,Locati M,Kim YO,Weissman D,Murphy PM||The Journal of biological chemistry (276:40133)||2001|
CD4 antigen (p55); CD4 antigen p55; CD4 receptor; T-cell surface antigen T4/Leu-3