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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||PBS with 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 3 publications below|
Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
Analyte Specific Reagent
CD4+CD25(high), CD8+CD28- cells and thyroid autoantibodies in breast cancer patients.
MHCD0401 was used in flow cytometry to study T cell subtypes in breast cancer patients with and without high levels of autoimmune thyroid antibodies.
|Biylgi O,Karagöz B,Türken O,Gültepe M,Özgün A,Tunçel T,Emirzeoğlu L,Çelik S,Müftüoğlu T,Gökhan Kandemir E||Central-European journal of immunology / Polish Society for Immunology and eleven other Central-European immunological societies (39:338)||2015|
The decidual stromal cells-secreted CCL2 induces and maintains decidual leukocytes into Th2 bias in human early pregnancy.
MHCD0401 was used in flow cytometry to study the roles of the decidua-derived CCL2 in the Th2 predominance found at the maternal-fetal interface.
|He YY,He XJ,Guo PF,Du MR,Shao J,Li MQ,Li DJ||Clinical immunology (Orlando, Fla.) (145:161)||2012|
Optimization of gene expression in nonactivated circulating lymphocytes.
MHCD0401 was used in flow cytometry to utilize systemic liposomes to increase the transfection and gene expression in circulating lymphocytes without activation.
|Zhang Y,Lu H,LiWang P,Sili U,Templeton NS||Molecular therapy : the journal of the American Society of Gene Therapy (8:629)||2003|
CD4 antigen (p55), CD4 receptor, T-cell surface antigen T4/Leu-3, CD4 antigen p55