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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||PBS with 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
Analyte Specific Reagent
Zoster Vaccination Increases the Breadth of CD4+ T Cells Responsive to Varicella Zoster Virus.
MHCD0401 was used in flow cytometry to measure the memory CD4(+) T-cell responses to each varicella zoster virus protein at baseline and after zoster vaccination.
|Laing KJ,Russell RM,Dong L,Schmid DS,Stern M,Magaret A,Haas JG,Johnston C,Wald A,Koelle DM||The Journal of infectious diseases (212:1022)||2015|
Flow cytometry in the bone marrow staging of mature B-cell neoplasms.
MHCD0401 was used in flow cytometry to ask if flow cytometry can be used for the detection of bone marrow involvement in the different types of B-cell malignant neoplasms.
|Stacchini A,Demurtas A,Godio L,Martini G,Antinoro V,Palestro G||Cytometry. Part B, Clinical cytometry (54:10)||2003|
Gammadelta+ T cells preferentially respond to live rather than killed malaria parasites.
MHCD0401 was used in flow cytometry to study responses of peripheral blood T cells to malaria antigens.
|Waterfall M,Black A,Riley E||Infection and immunity (66:2393)||1998|
CD4+CD25(high), CD8+CD28- cells and thyroid autoantibodies in breast cancer patients.
MHCD0401 was used in flow cytometry to study T cell subtypes in breast cancer patients with and without high levels of autoimmune thyroid antibodies.
|Biylgi O,Karagöz B,Türken O,Gültepe M,Özgün A,Tunçel T,Emirzeo¿lu L,Çelik S,Müftüo¿lu T,Gökhan Kandemir E||Central-European journal of immunology (39:338)||2015|
The decidual stromal cells-secreted CCL2 induces and maintains decidual leukocytes into Th2 bias in human early pregnancy.
MHCD0401 was used in flow cytometry to study the roles of the decidua-derived CCL2 in the Th2 predominance found at the maternal-fetal interface.
|He YY,He XJ,Guo PF,Du MR,Shao J,Li MQ,Li DJ||Clinical immunology (Orlando, Fla.) (145:161)||2012|
In vivo inhibition of human CD19-targeted effector T cells by natural T regulatory cells in a xenotransplant murine model of B cell malignancy.
MHCD0401 was used in flow cytometry to examine the impact of Tregs on CAR-modified T cells in the SCID-Beige xenotransplant model.
|Lee JC,Hayman E,Pegram HJ,Santos E,Heller G,Sadelain M,Brentjens R||Cancer research (71:2871)||2011|
Optimization of gene expression in nonactivated circulating lymphocytes.
MHCD0401 was used in flow cytometry to utilize systemic liposomes to increase the transfection and gene expression in circulating lymphocytes without activation.
|Zhang Y,Lu H,LiWang P,Sili U,Templeton NS||Molecular therapy : the journal of the American Society of Gene Therapy (8:629)||2003|
CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4