Flow cytometry histogram showing human mononuclear cells stained with anti-CD4 complexed with Alexa Fluor® 700 Zenon® labeling reagent. An antibody complex was made by using the Zenon® Alexa Fluor® 700 Mouse IgG1 Labeling Kit (Cat. no. Z25011) and mouse anti human CD4. Human mononuclear cells were stained with the antibody complex and analyzed by flow cytometry using a 633 nm laser.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||PBS with BSA, sucrose|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.
This antibody may be used, in combination with other indicators, for the diagnosis or prognosis of immunodeficiency diseases, including hypogammaglobulinemia, severe combined immunodeficiency (SCID), and the acquired immunodeficiency disease (AIDS).
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
Class 2 Device
Nef neutralizes the ability of exosomes from CD4+ T cells to act as decoys during HIV-1 infection.
MHCD0404-4 was used in flow cytometry and immunocytochemistry to test if Nef modifies the composition of exosomes released by T lymphocytes.
|de Carvalho JV,de Castro RO,da Silva EZ,Silveira PP,da Silva-Januário ME,Arruda E,Jamur MC,Oliver C,Aguiar RS,daSilva LL||PloS one (9:null)||2014|
Primary B-cell deficiencies reveal a link between human IL-17-producing CD4 T-cell homeostasis and B-cell differentiation.
MHCD0404-4 was used in flow cytometry to elucidate the link between Th17-cell homeostasis and B cells.
|Barbosa RR,Silva SP,Silva SL,Melo AC,Pedro E,Barbosa MP,Pereira-Santos MC,Victorino RM,Sousa AE||PloS one (6:null)||2011|
T cell priming enhances IL-4 gene expression by increasing nuclear factor of activated T cells.
MHCD0404-4 was used in flow cytometry to study selective expression of cytokines by T cells.
|Cron RQ,Bort SJ,Wang Y,Brunvand MW,Lewis DB||Journal of immunology (Baltimore, Md. : 1950) (162:860)||1999|