|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||PBS with BSA, sucrose|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The TRI-COLOR® conjugate is a tandem R-phycoerythrin (PE)-Cy5® conjugate that permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser.
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
Analyte Specific Reagent
Intermediate DNA methylation is a conserved signature of genome regulation.
MHCD0406 was used in flow cytometry to show that intermediate DNA methylation is a conserved signature of gene regulation and exon usage
|Elliott G,Hong C,Xing X,Zhou X,Li D,Coarfa C,Bell RJ,Maire CL,Ligon KL,Sigaroudinia M,Gascard P,Tlsty TD,Harris RA,Schalkwyk LC,Bilenky M,Mill J,Farnham PJ,Kellis M,Marra MA,Milosavljevic A,Hirst M,Stormo GD,Wang T,Costello JF||Nature communications (6:null)||2015|
Changes in the proportions of CD4(+)T cell subsets defined by CD127 and CD25 expression during HBV infection.
MHCD0406 was used in flow cytometry to study changes in T cell subsets during HBV infection.
|Xu HT,Ye J,Chen YB,Zhang LX,Huang JX,Xian JC,Liu L,Peng HL,Li L,Lin M,Huang JH||Immunological investigations (41:290)||2012|
Analysis of T-cell clones in systemic lupus erythematosus.
MHCD0406 was used in flow cytometry to investigate the role Th cells and their relationship with Th2 cells in human systemic lupus erythematosus.
|Converso M,Bertero MT,Vallario A,Caligaris-Cappio F||Haematologica (85:118)||2000|
Cytokine signatures of innate and adaptive immunity in 17DD yellow fever vaccinated children and its association with the level of neutralizing antibody.
MHCD0406 was used in flow cytometry to discuss the live attenuated yellow fever vaccines.
|Luiza-Silva M,Campi-Azevedo AC,Batista MA,Martins MA,Avelar RS,da Silveira Lemos D,Bastos Camacho LA,de Menezes Martins R,de Lourdes de Sousa Maia M,Guedes Farias RH,da Silva Freire M,Galler R,Homma A,Leite Ribeiro JG,Campos Lemos JA,Auxiliadora-Martins M,Eloi-Santos SM,Teixeira-Carvalho A,Martins-Filho OA||The Journal of infectious diseases (204:873)||2011|
Human CD4+CD25+ regulatory cells have marked and sustained effects on CD8+ T cell activation.
MHCD0406 was used in flow cytometry to study the interactions between CD4(+)CD25(+) T cells and human CD8(+) T cells.
|Câmara NO,Sebille F,Lechler RI||European journal of immunology (33:3473)||2003|
Induction of allopeptide-specific human CD4+CD25+ regulatory T cells ex vivo.
MHCD0406 was used in flow cytometry to investigate antigen specificity of CD4+CD25+ regulatory T cells.
|Jiang S,Camara N,Lombardi G,Lechler RI||Blood (102:2180)||2003|
Expression of CD4 on human peripheral blood neutrophils.
MHCD0406 was used in flow cytometry to report the unconventional expression of CD4 at the surface of peripheral blood neutrophils.
|Biswas P,Mantelli B,Sica A,Malnati M,Panzeri C,Saccani A,Hasson H,Vecchi A,Saniabadi A,Lusso P,Lazzarin A,Beretta A||Blood (101:4452)||2003|
Reduced cell surface expression of CCR5 in CCR5Delta 32 heterozygotes is mediated by gene dosage, rather than by receptor sequestration.
MHCD0406 was used in flow cytometry to determine how CCR5 expression is reduced in cells from ccr5Delta32 heterozygotes.
|Venkatesan S,Petrovic A,Van Ryk DI,Locati M,Weissman D,Murphy PM||The Journal of biological chemistry (277:2287)||2002|
A membrane-proximal basic domain and cysteine cluster in the C-terminal tail of CCR5 constitute a bipartite motif critical for cell surface expression.
MHCD0406 was used in flow cytometry to characterize CCR5 truncations.
|Venkatesan S,Petrovic A,Locati M,Kim YO,Weissman D,Murphy PM||The Journal of biological chemistry (276:40133)||2001|
Expression and coreceptor activity of STRL33/Bonzo on primary peripheral blood lymphocytes.
MHCD0406 was used in flow cytometry to characterize the localization and function of STRL33/Bonzo.
|Sharron M,Pöhlmann S,Price K,Lolis E,Tsang M,Kirchhoff F,Doms RW,Lee B||Blood (96:41)||2000|
Retrovirally transduced CD34++ human cord blood cells generate T cells expressing high levels of the retroviral encoded green fluorescent protein marker in vitro.
MHCD0406 was used in flow cytometry to report an in vitro assay for thymic T-cell development.
|Verhasselt B,De Smedt M,Verhelst R,Naessens E,Plum J||Blood (91:431)||1998|