|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||PBS with 10% sucrose, BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The R-phycoerythrin (PE)-Texas Red® conjugate permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser.
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
Analyte Specific Reagent
Optimization of the cytokine secretion assay for human IL-2 in single and combination assays.
MHCD0417 was used in flow cytometry to optimize detection of IL-2 from live cells
|Deng N,Mosmann TR||Cytometry. Part A : the journal of the International Society for Analytical Cytology (87:777)||2015|
Plasmacytoid dendritic cells suppress HIV-1 replication but contribute to HIV-1 induced immunopathogenesis in humanized mice.
MHCD0417 was used in flow cytometry to study the role of plasmacytoid dendritic cells in human immunodeficiency virus type 1 infection and pathogenesis
|Li G,Cheng M,Nunoya J,Cheng L,Guo H,Yu H,Liu YJ,Su L,Zhang L||PLoS pathogens (10:null)||2014|
Isolation and cytokine analysis of lamina propria lymphocytes from mucosal biopsies of the human colon.
MHCD0417 was used in flow cytometry to analyze mucosal pinch biopsies collected predominantly during colonoscopies.
|Bowcutt R,Malter LB,Chen LA,Wolff MJ,Robertson I,Rifkin DB,Poles M,Cho I,Loke P||Journal of immunological methods (421:27)||2015|
High numbers of differentiated effector CD4 T cells are found in patients with cancer and correlate with clinical response after neoadjuvant therapy of breast cancer.
MHCD0417 was used in flow cytometry to examine human differentiated effector CD4(+) T cells that are defined by low levels of IL-2 and IL-7 receptors.
|Péguillet I,Milder M,Louis D,Vincent-Salomon A,Dorval T,Piperno-Neumann S,Scholl SM,Lantz O||Cancer research (74:2204)||2014|
B cell responses to HIV antigen are a potent correlate of viremia in HIV-1 infection and improve with PD-1 blockade.
MHCD0417 was used in flow cytometry to assess the extent of B cell dysfunction during HIV infection.
|Nicholas KJ,Zern EK,Barnett L,Smith RM,Lorey SL,Copeland CA,Sadagopal S,Kalams SA||PloS one (8:null)||2013|
Ki-67 expression reveals strong, transient influenza specific CD4 T cell responses after adult vaccination.
MHCD0417 was used in flow cytometry to study influenza-specific CD4 T cells respond to vaccination using Ki67 as a marker.
|Li X,Miao H,Henn A,Topham DJ,Wu H,Zand MS,Mosmann TR||Vaccine (30:4581)||2012|
CD8+ T cell immunity to 2009 pandemic and seasonal H1N1 influenza viruses.
MHCD0417 was used in flow cytometry to examine human cross-reactive T cells against a pandemic virus.
|Scheible K,Zhang G,Baer J,Azadniv M,Lambert K,Pryhuber G,Treanor JJ,Topham DJ||Vaccine (29:2159)||2011|
Peripheral immature CD2-/low T cell development from type 2 to type 1 cytokine production.
MHCD0417 was used in flow cytometry to identify and characterize immature CD2(-/low) T cells in peripheral blood.
|Loza MJ,Perussia B||Journal of immunology (Baltimore, Md. : 1950) (169:3061)||2002|