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Human peripheral blood lymphocytes were stained using PE-Cy®5.5 of anti-human CD4 monoclonal antibody (clone S3.5). The negative control profiles represent unstained cells.
| Details | |
|---|---|
| Tested species reactivity | Human |
| Published species reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S3.5 |
| Immunogen | Human CD4 |
| Conjugate | PE-Cy5.5® |
| Form | Liquid |
| Purification | purified |
| Storage buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4° C, store in dark |
| Tested Applications | Dilution * |
|---|---|
| Flow Cytometry (Flow) | Assay-Dependent |
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
| Published Applications | |
|---|---|
| Flow Cytometry (Flow) | See 1 publications below |
The R-phycoerythrin (PE)-Cy5.5® tandem conjugate permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser.
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
Analyte Specific Reagent
| Species | Dilution | Title | Authors | Journal (Vol:Pg) | Year |
|---|---|---|---|---|---|
| Human | Not Cited |
Capsid-specific T-cell responses to natural infections with adeno-associated viruses in humans differ from those of nonhuman primates.
MHCD0418 was used in flow cytometry to test if rejection of AAV-transduced hepatocytes is due to AAV capsid-specific CD8 positive T cells that become reactivated upon gene transfer. |
Li H,Lasaro MO,Jia B,Lin SW,Haut LH,High KA,Ertl HC | Molecular therapy : the journal of the American Society of Gene Therapy (19:2021) | 2011 |
CD4 antigen (p55), CD4 receptor, T-cell surface antigen T4/Leu-3, CD4 antigen p55
CD4mut
Signal transduction
Cell surface receptor mediated signal transduction
Immunity and defense
T-cell mediated immunity
MHCII-mediated immunity
Developmental processes
Mesoderm development
Hematopoiesis
Receptor
Defense/immunity protein
Immunoglobulin receptor family member
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