Invitrogen

CD4 Monoclonal Antibody (S3.5), PE-Cyanine5.5

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View Details

8 Published Figures

19 References

View all (452) CD4 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

CD4 Antibody in Flow Cytometry (Flow) — Human peripheral blood lymphocytes were stained using PE-Cy®5.5 of anti-human CD4 monoclonal antibody (clone S3.5). The negative control profiles represent unstained cells.

CD4 Antibody — Fig 4 PB gut-homing CD8+ T activation remains elevated in long-term ART experienced individuals on protease inhibitors. Frequencies (expressed as percentages) of peripheral blood (PB) memory gut homing (CD45RO+ CCR9+ B7+) CD4+ (A) and CD8+ (B) T-cells expressing both CD38 and HLADR was determined by flow cytometry on PBMCs of HIV-infected individuals (HIV+), antiretroviral (ART) naïve (n = 18), ART experienced individuals on short-term (n = 15) and long-term treatment (n = 32), HIV controllers (n = 6) and HIV seronegative individuals (SN, n = 5). Frequencies of PB memory gut homing CD8+ T-cells expressing the activation markers CD38 and HLADR in efavirenz (EFV) or protease inhibitors (PI) based therapy is shown in (C). Scatter plots were used to represent the data. Horizontal lines indicate median values. Each symbol represents one individual. The red symbols represent the females in each group. Groups were compared using Kruskal-Wallis test correcting for multiple comparisons using the Dunnett´s post-test. Only significant corrected p values are shown ***p<0.0005, **p<0.005, *p<0.05. Spearman's rank correlation between PB memory gut homing CD4+ (D) and CD8+ (E) T cell activation and plasma viral load (pVL) in the ART naive group. Gender was color-coded as follows: red dots, women and black dots, men. {TM}

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 17 publications 17 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

S3.5

Immunogen

Human CD4

Conjugate

PE-Cyanine5.5

Excitation/Emission Profile

View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

RRID

AB_10376013

Product Specific Information

The R-phycoerythrin (PE)-Cy5.5® tandem conjugate permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

Analyte Specific Reagent

Bioinformatics

Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

Gene Aliases: CD4; CD4mut

UniProt ID: (Human) P01730

Entrez Gene ID: (Human) 920

Function(s)