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      CD4 Monoclonal Antibody (S3.5), PE-Cyanine5.5

      See 263 other CD4 antibodies ›
      CD4 Monoclonal Antibody, PE-Cyanine5.5 conjugate for Flow

      Catalog#: MHCD0418 Invitrogen

      Catalog # Size Price (USD) Qty Datasheet
      MHCD0418 0.5 ml
      311.00
      Please enter a quantity in the range of 1 to 199.
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      Product Details

      References

      Bioinformatics

      Documents
      Details
      Tested species reactivity Human
      Published species reactivity Human, Rhesus monkey
      Host / Isotype Mouse / IgG2a
      Class Monoclonal
      Type Antibody
      Clone S3.5
      Immunogen Human CD4
      Conjugate PE-Cyanine5.5
      Form Liquid
      Purification purified
      Storage buffer PBS
      Contains 0.1% sodium azide
      Storage Conditions 4° C, store in dark
      Tested Applications Dilution *
      Flow Cytometry (Flow) Assay-Dependent

      * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.

      Published Applications
      Flow Cytometry (Flow) See 4 publications below
      Miscellaneous PubMed (MISC) See 2 publications below

      Product Specific Information

      The R-phycoerythrin (PE)-Cy5.5® tandem conjugate permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser.

      Background/Target Information

      The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

      Analyte Specific Reagent

      References

      Flow Cytometry

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Human Not Cited Selective reduction of IFN-¿ single positive mycobacteria-specific CD4+ T cells in HIV-1 infected individuals with latent tuberculosis infection.
      MHCD0418 was used in flow cytometry to explore how HIV-1 alters immunity during latent tuberculosis       		Riou C,Bunjun R,Müller TL,Kiravu A,Ginbot Z,Oni T,Goliath R,Wilkinson RJ,Burgers WA Tuberculosis (Edinburgh, Scotland) (101:25) 2016
      Human Not Cited Temporal Dynamics of CD8+ T Cell Effector Responses during Primary HIV Infection.
      MHCD0418 was used in flow cytometry to study the cytolytic effector capacity of HIV-specific CD8+ T cells       		Demers KR,Makedonas G,Buggert M,Eller MA,Ratcliffe SJ,Goonetilleke N,Li CK,Eller LA,Rono K,Maganga L,Nitayaphan S,Kibuuka H,Routy JP,Slifka MK,Haynes BF,McMichael AJ,Bernard NF,Robb ML,Betts MR PLoS pathogens (12:null) 2016
      Rhesus monkey Not Cited Human Immunodeficiency Virus Type 1 Monoclonal Antibodies Suppress Acute Simian-Human Immunodeficiency Virus Viremia and Limit Seeding of Cell-Associated Viral Reservoirs.
      MHCD0418 was used in flow cytometry to assess the ability of HIV-1 envelope glycoprotein-specific broadly neutralizing monoclonal antibodies to suppress acute simian-human immunodeficiency virus replication in rhesus macaques       		Bolton DL,Pegu A,Wang K,McGinnis K,Nason M,Foulds K,Letukas V,Schmidt SD,Chen X,Todd JP,Lifson JD,Rao S,Michael NL,Robb ML,Mascola JR,Koup RA Journal of virology (90:1321) 2015
      Human Not Cited Capsid-specific T-cell responses to natural infections with adeno-associated viruses in humans differ from those of nonhuman primates.
      MHCD0418 was used in flow cytometry to test if rejection of AAV-transduced hepatocytes is due to AAV capsid-specific CD8 positive T cells that become reactivated upon gene transfer.       		Li H,Lasaro MO,Jia B,Lin SW,Haut LH,High KA,Ertl HC Molecular therapy : the journal of the American Society of Gene Therapy (19:2021) 2011

      Miscellaneous PubMed

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Human Not Cited Effects of immunosuppression on circulating adeno-associated virus capsid-specific T cells in humans.
      MHCD0418 was used in immunocytochemistry to assess the effect of immunosuppression used for organ transplantation on immune responses to adeno-associated virus capsid antigens.       		Parzych EM,Li H,Yin X,Liu Q,Wu TL,Podsakoff GM,High KA,Levine MH,Ertl HC Human gene therapy (24:431) 2013
      Human Not Cited Anti-CD8 antibodies can trigger CD8+ T cell effector function in the absence of TCR engagement and improve peptide-MHCI tetramer staining.
      MHCD0418 was used in flow cytometry to test if anti-CD8 Abs induce effector function.       		Clement M,Ladell K,Ekeruche-Makinde J,Miles JJ,Edwards ES,Dolton G,Williams T,Schauenburg AJ,Cole DK,Lauder SN,Gallimore AM,Godkin AJ,Burrows SR,Price DA,Sewell AK,Wooldridge L Journal of immunology (Baltimore, Md. : 1950) (187:654) 2011

      Bioinformatics

      Protein Aliases:

      CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

      Gene Aliases:

      CD4; CD4mut

      UniProt ID:

      (Human) P01730

      Entrez Gene ID:

      (Human) 920

      Molecular Function:

      cytokine receptor defense/immunity protein immunoglobulin receptor superfamily receptor

      Function(s) Process(es)

      Documents

      Material safety data sheets (MSDS)

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