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|Tested species reactivity||Human|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG2a|
|Conjugate||Alexa Fluor® 488|
|Storage buffer||PBS with 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Based on our testing, publications, and results reported from customers, the Alexa Fluor® 488 dye provides the best fluorescein (FITC) substitute.
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
Analyte Specific Reagent
Polarized axonal surface expression of neuronal KCNQ potassium channels is regulated by calmodulin interaction with KCNQ2 subunit.
MHCD0420 was used in immunocytochemistry to identify the molecular mechanisms that regulate the enrichment of KCNQ channels at the neuronal axon
|Cavaretta JP,Sherer KR,Lee KY,Kim EH,Issema RS,Chung HJ||PloS one (9:null)||2014|
Cross-presentation of viral antigens in dribbles leads to efficient activation of virus-specific human memory T cells.
MHCD0420 was used in flow cytometry to study virus-specific human memory T-cell activation in response to Dribble-mediated cross-presentation of viral antigens.
|Ye W,Xing Y,Paustian C,van de Ven R,Moudgil T,Hilton TL,Fox BA,Urba WJ,Zhao W,Hu HM||Journal of translational medicine (12:null)||2014|
Microfluidic CD4+ and CD8+ T lymphocyte counters for point-of-care HIV diagnostics using whole blood.
MHCD0420 was used in flow cytometry to develop microfluidic biochips that count CD4(+) and CD8(+) lymphocytes in whole blood samples to use in third world countries.
|Watkins NN,Hassan U,Damhorst G,Ni H,Vaid A,Rodriguez W,Bashir R||Science translational medicine (5:null)||2013|
CD4 antigen (p55); CD4 antigen p55; CD4 receptor; T-cell surface antigen T4/Leu-3