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Flow cytometry histogram showing human mononuclear cells incubated with anti-CD8 complexed with biotin-XX Zenon® labeling reagent and followed by staining with Alexa Fluor® 488 streptavidin. An antibody complex was made by using the Zenon® Biotin-XX Mouse IgG2a Labeling Kit (Cat. no. Z25152) and mouse anti human CD8 (Cat. no. MHCD0800). Human mononuclear cells were incubated with the antibody complex, washed, stained with streptavidin, Alexa Fluor® 488 conjugate (Cat. no. S11223) and analyzed by flow cytometry using a 488 nm Laser.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. CD38 (T10) is a single chain 46 kDa type II integral glycoprotein with a short N terminal cytoplasmic tail. CD38 is highly expressed on thymocytes. It is also expressed by early cells of B and T lineages, NK cells, plasma cells, monocytes and macrophages and may be detected on cells from multiple myeloma, ALL (B and T) and some AML. In normal lymph nodes and tonsils the antigen is detected mainly on B cells in germinal centers and in plasma cells. The extracellular domain of the molecule shares a high homology sequence with Aplysia ADP ribosyl cyclase. CD38 functions as a multicatalytic ectoenzyme serving as ADP ribosyl cyclase, ADPR hydrolase and possibly NAD+ glycohydrolase, or as a surface receptor.
Analyte Specific Reagent
Multidimensional Clusters of CD4+ T Cell Dysfunction Are Primarily Associated with the CD4/CD8 Ratio in Chronic HIV Infection.
MHCD0800 was used in flow cytometry to assess the CD4/CD8 ratio as a marker for CD4+ T cell dysfunction in chronic HIV infection
|Frederiksen J,Buggert M,Noyan K,Nowak P,Sönnerborg A,Lund O,Karlsson AC||PloS one (10:null)||2015|
IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia.
MHCD0800 was used in flow cytometry to developed a strategy to expand umbilical cord blood T cells and test their effects after transplantation
|Pegram HJ,Purdon TJ,van Leeuwen DG,Curran KJ,Giralt SA,Barker JN,Brentjens RJ||Leukemia (29:415)||2015|
Comparative analysis of SIV-specific cellular immune responses induced by different vaccine platforms in rhesus macaques.
MHCD0800 was used in flow cytometry to compare five SIV vaccine platforms
|Valentin A,McKinnon K,Li J,Rosati M,Kulkarni V,Pilkington GR,Bear J,Alicea C,Vargas-Inchaustegui DA,Jean Patterson L,Pegu P,Liyanage NP,Gordon SN,Vaccari M,Wang Y,Hogg AE,Frey B,Sui Y,Reed SG,Sardesai NY,Berzofsky JA,Franchini G,Robert-Guroff M,Felber BK,Pavlakis GN||Clinical immunology (Orlando, Fla.) (155:91)||2014|
Microfluidic CD4+ and CD8+ T lymphocyte counters for point-of-care HIV diagnostics using whole blood.
MHCD0800 was used in flow cytometry to develop microfluidic biochips that count CD4(+) and CD8(+) lymphocytes in whole blood samples to use in third world countries.
|Watkins NN,Hassan U,Damhorst G,Ni H,Vaid A,Rodriguez W,Bashir R||Science translational medicine (5:null)||2013|
Coincidence detection of heterogeneous cell populations from whole blood with coplanar electrodes in a microfluidic impedance cytometer.
MHCD0800 was used in ChIP assay to characterize the coincidence detection with cell differentiation using a microfluidic impedance biochip
|Hassan U,Bashir R||Lab on a chip (14:4370)||2014|
Reduced cell surface expression of CCR5 in CCR5Delta 32 heterozygotes is mediated by gene dosage, rather than by receptor sequestration.
MHCD0800 was used in flow cytometry to determine how CCR5 expression is reduced in cells from ccr5Delta32 heterozygotes.
|Venkatesan S,Petrovic A,Van Ryk DI,Locati M,Weissman D,Murphy PM||The Journal of biological chemistry (277:2287)||2002|
A membrane-proximal basic domain and cysteine cluster in the C-terminal tail of CCR5 constitute a bipartite motif critical for cell surface expression.
MHCD0800 was used in flow cytometry to characterize CCR5 truncations.
|Venkatesan S,Petrovic A,Locati M,Kim YO,Weissman D,Murphy PM||The Journal of biological chemistry (276:40133)||2001|
CD8; CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; Leu-2; Leu2 T-lymphocyte antigen; MAL; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen
CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37