Dual color flow cytometry dot plot showing mouse anti human CD8 RPE vs. mouse anti human CD4 biotin followed by streptavidin RPE-Alexa Fluor® 610. Human mononuclear cells were stained with mouse anti human CD4 biotin (Cat. no. MHCD0415), washed, stained with streptavidin RPE-Alexa Fluor® 610 (Cat. no. S20982) and mouse anti human CD8 RPE (Cat. no. MHCD08044), washed, and analyzed on a flow cytometer with a 488 nm excitation source.
|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||PBS with BSA, sucrose|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.
CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. CD38 (T10) is a single chain 46 kDa type II integral glycoprotein with a short N terminal cytoplasmic tail. CD38 is highly expressed on thymocytes. It is also expressed by early cells of B and T lineages, NK cells, plasma cells, monocytes and macrophages and may be detected on cells from multiple myeloma, ALL (B and T) and some AML. In normal lymph nodes and tonsils the antigen is detected mainly on B cells in germinal centers and in plasma cells. The extracellular domain of the molecule shares a high homology sequence with Aplysia ADP ribosyl cyclase. CD38 functions as a multicatalytic ectoenzyme serving as ADP ribosyl cyclase, ADPR hydrolase and possibly NAD+ glycohydrolase, or as a surface receptor.
Class 2 Device
IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia.
MHCD0804-4 was used in flow cytometry to developed a strategy to expand umbilical cord blood T cells and test their effects after transplantation.
|Pegram HJ,Purdon TJ,van Leeuwen DG,Curran KJ,Giralt SA,Barker JN,Brentjens RJ||Leukemia (29:415)||2015|
Flow cytometry in the bone marrow staging of mature B-cell neoplasms.
MHCD0804-4 was used in flow cytometry to ask if flow cytometry can be used for the detection of bone marrow involvement in the different types of B-cell malignant neoplasms.
|Stacchini A,Demurtas A,Godio L,Martini G,Antinoro V,Palestro G||Cytometry. Part B, Clinical cytometry (54:10)||2003|
Reactive oxygen intermediates during programmed cell death induced in the thymus of the Ts(1716)65Dn mouse, a murine model for human Down's syndrome.
MHCD0804-4 was used in flow cytometry to elucidate role of SOD-1 overexpression in thymocyte biology.
|Paz-Miguel JE,Flores R,Sánchez-Velasco P,Ocejo-Vinyals G,Escribano de Diego J,López de Rego J,Leyva-Cobián F||Journal of immunology (Baltimore, Md. : 1950) (163:5399)||1999|
Gammadelta+ T cells preferentially respond to live rather than killed malaria parasites.
MHCD0804-4 was used in flow cytometry to study responses of peripheral blood T cells to malaria antigens.
|Waterfall M,Black A,Riley E||Infection and immunity (66:2393)||1998|