|Tested species reactivity||Human|
|Published species reactivity||Non-human primate, Human, Rhesus monkey|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||PBS with BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Our Pacific Orange™ dye is optimally excited by the violet laser, and it is recommended that a 575, 585 or 600 nm band pass filter be used for optimal detection. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.
This antibody recognizes the alpha chain alone as well as the alpha/beta heterodimer.
CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. CD38 (T10) is a single chain 46 kDa type II integral glycoprotein with a short N terminal cytoplasmic tail. CD38 is highly expressed on thymocytes. It is also expressed by early cells of B and T lineages, NK cells, plasma cells, monocytes and macrophages and may be detected on cells from multiple myeloma, ALL (B and T) and some AML. In normal lymph nodes and tonsils the antigen is detected mainly on B cells in germinal centers and in plasma cells. The extracellular domain of the molecule shares a high homology sequence with Aplysia ADP ribosyl cyclase. CD38 functions as a multicatalytic ectoenzyme serving as ADP ribosyl cyclase, ADPR hydrolase and possibly NAD+ glycohydrolase, or as a surface receptor.
Analyte Specific Reagent
Multidimensional Clusters of CD4+ T Cell Dysfunction Are Primarily Associated with the CD4/CD8 Ratio in Chronic HIV Infection.
MHCD0830 was used in flow cytometry to assess the CD4/CD8 ratio as a marker for CD4+ T cell dysfunction in chronic HIV infection
|Frederiksen J,Buggert M,Noyan K,Nowak P,Sönnerborg A,Lund O,Karlsson AC||PloS one (10:null)||2015|
IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia.
MHCD0830 was used in flow cytometry to developed a strategy to expand umbilical cord blood T cells and test their effects after transplantation
|Pegram HJ,Purdon TJ,van Leeuwen DG,Curran KJ,Giralt SA,Barker JN,Brentjens RJ||Leukemia (29:415)||2015|
Comparative analysis of SIV-specific cellular immune responses induced by different vaccine platforms in rhesus macaques.
MHCD0830 was used in flow cytometry to compare five SIV vaccine platforms
|Valentin A,McKinnon K,Li J,Rosati M,Kulkarni V,Pilkington GR,Bear J,Alicea C,Vargas-Inchaustegui DA,Jean Patterson L,Pegu P,Liyanage NP,Gordon SN,Vaccari M,Wang Y,Hogg AE,Frey B,Sui Y,Reed SG,Sardesai NY,Berzofsky JA,Franchini G,Robert-Guroff M,Felber BK,Pavlakis GN||Clinical immunology (Orlando, Fla.) (155:91)||2014|
The stromal cell antigen CD248 (endosialin) is expressed on naive CD8+ human T cells and regulates proliferation.
MHCD0830 was used in flow cytometry to report that CD248 controls CD8(+) human T cell proliferation.
|Hardie DL,Baldwin MJ,Naylor A,Haworth OJ,Hou TZ,Lax S,Curnow SJ,Willcox N,MacFadyen J,Isacke CM,Buckley CD||Immunology (133:288)||2011|
Restoration of CD4+ Responses to Copathogens in HIV-Infected Individuals on Antiretroviral Therapy Is Dependent on T Cell Memory Phenotype.
MHCD0830 was used in flow cytometry to study the dynamics of Ag-specific CD4(+) T cells during antiretroviral therapy.
|Riou C,Tanko RF,Soares AP,Masson L,Werner L,Garrett NJ,Samsunder N,Abdool Karim Q,Abdool Karim SS,Burgers WA||Journal of immunology (Baltimore, Md. : 1950) (195:2273)||2015|
Variation in Gamma-Globin Expression before and after Induction with Hydroxyurea Associated with BCL11A, KLF1 and TAL1.
MHCD0830 was used in flow cytometry to elucidate regulation of γ-globin expression in F-cells.
|Grieco AJ,Billett HH,Green NS,Driscoll MC,Bouhassira EE||PloS one (10:null)||2015|
|Rhesus monkey||Not Cited||
Effect of chronic morphine administration on circulating T cell population dynamics in rhesus macaques.
MHCD0830 was used in flow cytometry and western blot to investigate how chronic morphine exposure affects T cell numbers and functions.
|Cornwell WD,Lewis MG,Fan X,Rappaport J,Rogers TJ||Journal of neuroimmunology (265:43)||2013|
Phenotype and function of T cells infiltrating visceral metastases from gastrointestinal cancers and melanoma: implications for adoptive cell transfer therapy.
MHCD0830 was used in flow cytometry to examine tumor-infiltrating lymphocytes derived from liver and lung metastases from patients with gastrointestinal cancers or melanoma.
|Turcotte S,Gros A,Hogan K,Tran E,Hinrichs CS,Wunderlich JR,Dudley ME,Rosenberg SA||Journal of immunology (Baltimore, Md. : 1950) (191:2217)||2013|
Trichosanthin-stimulated dendritic cells induce a type 2 helper T lymphocyte response through the OX40 ligand.
MHCD0830 was used in flow cytometry to assess the effects of trichosanthin treatment.
|Yan R,Zhong W,Zhu Y,Zhang X||Journal of investigational allergology and clinical immunology (22:491)||2013|
Expression of integrin ¿2 receptor in human cord blood CD34+CD38-CD90+ stem cells engrafting long-term in NOD/SCID-IL2R¿(c) null mice.
MHCD0830 was used in flow cytometry to identify integrin α2 as a novel stem cell marker.
|Wong WM,Sigvardsson M,Åstrand-Grundström I,Hogge D,Larsson J,Qian H,Ekblom M||Stem cells (Dayton, Ohio) (31:360)||2013|
Vitamin B6 and the immunity in kidney transplant recipients.
MHCD0830 was used in flow cytometry to explore the importance of vitamin B6 in the recipients of allogeneic kidney graft.
|Jankowska M,Marsza¿¿ M,D¿bska-¿lizie¿ A,Carrero JJ,Lindholm B,Czarnowski W,Rutkowski B,Trzonkowski P||Journal of renal nutrition : the official journal of the Council on Renal Nutrition of the National Kidney Foundation (23:57)||2013|
HIV-induced changes in T cell signaling pathways.
MHCD0830 was used in flow cytometry to examine the changes in signaling pathways of human CD4(+) and CD8(+) T cells from HIV-infected patients.
|Schweneker M,Favre D,Martin JN,Deeks SG,McCune JM||Journal of immunology (Baltimore, Md. : 1950) (180:6490)||2008|
|Non-human primate||Not Cited||
Flow cytometry identification and characterization of mononuclear cell subsets in the neotropical primate Saimiri sciureus (squirrel monkey).
MHCD0830 was used in flow cytometry to discuss available reagents for studying the neotropical primate squirrel monkey.
|Contamin H,Loizon S,Bourreau E,Michel JC,Garraud O,Mercereau-Puijalon O,Behr C||Journal of immunological methods (297:61)||2005|
Screening for T cell-eliciting proteins of Japanese encephalitis virus in a healthy JE-endemic human cohort using recombinant baculovirus-infected insect cell preparations.
MHCD0830 was used in flow cytometry to report the adaptive T cell responses to Japanese encephalitis virus in a naturally exposed healthy virus-immune human cohort.
|Kumar P,Uchil PD,Sulochana P,Nirmala G,Chandrashekar R,Haridattatreya M,Satchidanandam V||Archives of virology (148:1569)||2003|
Trafficking machinery of NKT cells: shared and differential chemokine receptor expression among V alpha 24(+)V beta 11(+) NKT cell subsets with distinct cytokine-producing capacity.
MHCD0830 was used in flow cytometry to report differential expression of chemokine receptors among NKT cell subsets.
|Kim CH,Johnston B,Butcher EC||Blood (100:11)||2002|
Cryptomonad algal phycobiliproteins as fluorochromes for extracellular and intracellular antigen detection by flow cytometry.
MHCD0830 was used in flow cytometry to assess cryptomonad-derived phycobiliproteins for their applicability to flow cytometry.
|Telford WG,Moss MW,Morseman JP,Allnutt FC||Cytometry (44:16)||2001|
Expression and coreceptor activity of STRL33/Bonzo on primary peripheral blood lymphocytes.
MHCD0830 was used in flow cytometry to characterize the localization and function of STRL33/Bonzo.
|Sharron M,Pöhlmann S,Price K,Lolis E,Tsang M,Kirchhoff F,Doms RW,Lee B||Blood (96:41)||2000|
CK beta-11/macrophage inflammatory protein-3 beta/EBI1-ligand chemokine is an efficacious chemoattractant for T and B cells.
MHCD0830 was used in flow cytometry to determine the functional properties of CK beta-11/MIP-3 beta/ELC.
|Kim CH,Pelus LM,White JR,Applebaum E,Johanson K,Broxmeyer HE||Journal of immunology (Baltimore, Md. : 1950) (160:2418)||1998|