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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||PBS with BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 3 publications below|
Our Pacific Orange™ dye is optimally excited by the violet laser, and it is recommended that a 575, 585 or 600 nm band pass filter be used for optimal detection. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.
This antibody recognizes the alpha chain alone as well as the alpha/beta heterodimer.
CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. CD38 (T10) is a single chain 46 kDa type II integral glycoprotein with a short N terminal cytoplasmic tail. CD38 is highly expressed on thymocytes. It is also expressed by early cells of B and T lineages, NK cells, plasma cells, monocytes and macrophages and may be detected on cells from multiple myeloma, ALL (B and T) and some AML. In normal lymph nodes and tonsils the antigen is detected mainly on B cells in germinal centers and in plasma cells. The extracellular domain of the molecule shares a high homology sequence with Aplysia ADP ribosyl cyclase. CD38 functions as a multicatalytic ectoenzyme serving as ADP ribosyl cyclase, ADPR hydrolase and possibly NAD+ glycohydrolase, or as a surface receptor.
Analyte Specific Reagent
Multidimensional Clusters of CD4+ T Cell Dysfunction Are Primarily Associated with the CD4/CD8 Ratio in Chronic HIV Infection.
MHCD0830 was used in flow cytometry to assess the CD4/CD8 ratio as a marker for CD4+ T cell dysfunction in chronic HIV infection
|Frederiksen J,Buggert M,Noyan K,Nowak P,Sönnerborg A,Lund O,Karlsson AC||PloS one (10:null)||2015|
IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia.
MHCD0830 was used in flow cytometry to developed a strategy to expand umbilical cord blood T cells and test their effects after transplantation
|Pegram HJ,Purdon TJ,van Leeuwen DG,Curran KJ,Giralt SA,Barker JN,Brentjens RJ||Leukemia (29:415)||2015|
Comparative analysis of SIV-specific cellular immune responses induced by different vaccine platforms in rhesus macaques.
MHCD0830 was used in flow cytometry to compare five SIV vaccine platforms
|Valentin A,McKinnon K,Li J,Rosati M,Kulkarni V,Pilkington GR,Bear J,Alicea C,Vargas-Inchaustegui DA,Jean Patterson L,Pegu P,Liyanage NP,Gordon SN,Vaccari M,Wang Y,Hogg AE,Frey B,Sui Y,Reed SG,Sardesai NY,Berzofsky JA,Franchini G,Robert-Guroff M,Felber BK,Pavlakis GN||Clinical immunology (Orlando, Fla.) (155:91)||2014|
CD8 antigen, alpha polypeptide (p32), Leu2 T-lymphocyte antigen, OKT8 T-cell antigen, T cell co-receptor, T-cell antigen Leu2, T-lymphocyte differentiation antigen T8/Leu-2, T8 T-cell antigen, CD8 antigen, beta polypeptide 1 (p37), T lymphocyte surface glycoprotein beta chain, CD8, Leu-2, MAL, CD8a, CD8b, CD8alpha, CD8beta
Leu2, LEU2, P37, CD8, LY3, MAL, CD8B1, p32, LYT3
Signal transduction Cell surface receptor mediated signal transduction Receptor protein tyrosine kinase signaling pathway Immunity and defense T-cell mediated immunity MHCI-mediated immunity Other receptor mediated signaling pathway