Histogram of gated human lymphocytes labeled with CD8 Mouse Anti-Human mAb (clone 3B5), Qdot® 800 Conjugate Conjugate. Sample was acquired and analyzed using 405 nm excitation and a 780/60 band pass emission filter using a BD LSR II flow cytometer (BD Biosciences, San Jose, CA).
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recognizes the CD8 antigen|
|Storage buffer||0.05M borate, pH 8.3, with 1M betaine|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 2 publications below|
CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. CD38 (T10) is a single chain 46 kDa type II integral glycoprotein with a short N terminal cytoplasmic tail. CD38 is highly expressed on thymocytes. It is also expressed by early cells of B and T lineages, NK cells, plasma cells, monocytes and macrophages and may be detected on cells from multiple myeloma, ALL (B and T) and some AML. In normal lymph nodes and tonsils the antigen is detected mainly on B cells in germinal centers and in plasma cells. The extracellular domain of the molecule shares a high homology sequence with Aplysia ADP ribosyl cyclase. CD38 functions as a multicatalytic ectoenzyme serving as ADP ribosyl cyclase, ADPR hydrolase and possibly NAD+ glycohydrolase, or as a surface receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Selective Loss of Early Differentiated, Highly Functional PD1high CD4 T Cells with HIV Progression.
Q22157 was used in flow cytometry to analyze HIV progression traits of a selective loss of early differentiated and highly functional PD1high CD4 T cells
|Paris RM,Petrovas C,Ferrando-Martinez S,Moysi E,Boswell KL,Archer E,Yamamoto T,Ambrozak D,Casazza JP,Haubrich R,Connors M,Ake J,Kim JH,Koup RA||PloS one (10:null)||2015|
|Not Applicable||Not Cited||
Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class I tetramers.
Q22157 was used in flow cytometry to study low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class 1 tetramers and techniques to improve the direct ex vivo detection
|Chattopadhyay PK,Melenhorst JJ,Ladell K,Gostick E,Scheinberg P,Barrett AJ,Wooldridge L,Roederer M,Sewell AK,Price DA||Cytometry. Part A : the journal of the International Society for Analytical Cytology (73:1001)||2008|