Invitrogen

CD8a Monoclonal Antibody (RPA-T8), PE-Cyanine7, eBioscience™

Product Image Gallery

CD8a Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human CD8a PE-Cyanine7 (filled histogram). Cells in the lymphocyte gate were used for analysis.

Product Details

25-0088-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.125 µg)/test	View 26 publications 26 publications

Product Specifications
Species Reactivity	Human
Published species	Human, Non-human primate, Rat
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	RPA-T8
Conjugate	PE-Cyanine7 Alexa Fluor 532 Alexa Fluor 561 Alexa Fluor 660 Alexa Fluor 700 APC APC-eFluor 780 Brilliant UV 737 Brilliant UV 805 eFluor 450 eFluor 506 FITC PE PE-Cyanine5 PE-Cyanine5.5 PE-eFluor 610 PerCP-Cyanine5.5 Super Bright 436 Super Bright 600 Super Bright 645 Super Bright 702 Super Bright 780
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_1659702

Product Specific Information

Description: The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.

Applications Reported: This RPA-T8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This RPA-T8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Umbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria.

Stem cells international

Chen J,Wang Q,Feng X,Zhang Z,Geng L,Xu T,Wang D,Sun L

Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

A Cross-Reactive Small Protein Binding Domain Provides a Model to Study Off-Tumor CAR-T Cell Toxicity.

Molecular therapy oncolytics

Hammill JA,Kwiecien JM,Dvorkin-Gheva A,Lau VWC,Baker C,Wu Y,Bezverbnaya K,Aarts C,Heslen CW,Denisova GF,Derocher H,Milne K,Nelson BH,Bramson JL

Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry

Fri Jun 26 00:00:00 EDT 2020

Niraparib activates interferon signaling and potentiates anti-PD-1 antibody efficacy in tumor models.

Scientific reports

Wang Z,Sun K,Xiao Y,Feng B,Mikule K,Ma X,Feng N,Vellano CP,Federico L,Marszalek JR,Mills GB,Hanke J,Ramaswamy S,Wang J

25-0088-42 was used in Flow Cytometry to investigate the therapeutic potential of combining niraparib with anti-P-1 immune checkpoint inhibitors in preclinical tumour models.

Tue Feb 12 00:00:00 EST 2019

Immunological Properties of Neural Crest Cells Derived from Human Induced Pluripotent Stem Cells.

Stem cells and development

Fujii S,Yoshida S,Inagaki E,Hatou S,Tsubota K,Takahashi M,Shimmura S,Sugita S

Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

STAT3 Inhibition Combined with CpG Immunostimulation Activates Antitumor Immunity to Eradicate Genetically Distinct Castration-Resistant Prostate Cancers.

Clinical cancer research : an official journal of the American Association for Cancer Research

Moreira D,Adamus T,Zhao X,Su YL,Zhang Z,White SV,Swiderski P,Lu X,DePinho RA,Pal SK,Kortylewski M

25-0088 was used in Flow cytometry/Cell sorting to show a novel strategy combining STAT3 inhibition with Toll-like Receptor 9 (TLR9) stimulation to unleash immune response against prostate cancers regardless of the genetic background.

Sat Dec 01 00:00:00 EST 2018

Detection of Retinal Pigment Epithelium-Specific Antibody in iPSC-Derived Retinal Pigment Epithelium Transplantation Models.

Stem cell reports

Sugita S,Makabe K,Fujii S,Iwasaki Y,Kamao H,Shiina T,Ogasawara K,Takahashi M

Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry

Tue Nov 14 00:00:00 EST 2017

Two separate effects contribute to regulatory T cell defect in systemic lupus erythematosus patients and their unaffected relatives.

Clinical and experimental immunology

Costa N,Marques O,Godinho SI,Carvalho C,Leal B,Figueiredo AM,Vasconcelos C,Marinho A,Moraes-Fontes MF,Gomes da Costa A,Ponte C,Campanilho-Marques R,Cóias T,Martins AR,Viana JF,Lima M,Martins B,Fesel C

25-0088 was used in Flow cytometry/Cell sorting to determine the origin and role of the SLE Treg phenotype in systemic lupus erythematosus.

Fri Sep 01 00:00:00 EDT 2017

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors.

Stem cell reports

Sugita S,Iwasaki Y,Makabe K,Kimura T,Futagami T,Suegami S,Takahashi M

Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry

Tue Oct 11 00:00:00 EDT 2016

Follicular Regulatory CD8 T Cells Impair the Germinal Center Response in SIV and Ex Vivo HIV Infection.

PLoS pathogens

Miles B,Miller SM,Folkvord JM,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2016

CD28 family of receptors on T cells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade.

Molecular medicine reports

Tang ZS,Hao YH,Zhang EJ,Xu CL,Zhou Y,Zheng X,Yang DL

Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

B cells from African American lupus patients exhibit an activated phenotype.

JCI insight

Menard LC,Habte S,Gonsiorek W,Lee D,Banas D,Holloway DA,Manjarrez-Orduno N,Cunningham M,Stetsko D,Casano F,Kansal S,Davis PM,Carman J,Zhang CK,Abidi F,Furie R,Nadler SG,Suchard SJ

25-0088 was used in Flow cytometry/Cell sorting to investigate immunological differences between African American and European American patients with systemic lupus erythematosus, showing that B cells from African American patients exhibit an activated phenotype.

Thu Jun 16 00:00:00 EDT 2016

Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.

Journal of immunology (Baltimore, Md. : 1950)

Younis RH,Han KL,Webb TJ

25-0088 was used in Flow cytometry/Cell sorting to examine the role of human head and neck squamous cell carcinoma-secreted Sema4D on myeloid cell differentiation.

Mon Feb 01 00:00:00 EST 2016

Investigating the causes for decreased levels of glutathione in individuals with type II diabetes.

PloS one

Lagman M,Ly J,Saing T,Kaur Singh M,Vera Tudela E,Morris D,Chi PT,Ochoa C,Sathananthan A,Venketaraman V

Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry

Wed Dec 30 00:00:00 EST 2015

RSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection.

Nature communications

Jozwik A,Habibi MS,Paras A,Zhu J,Guvenel A,Dhariwal J,Almond M,Wong EHC,Sykes A,Maybeno M,Del Rosario J,Trujillo-Torralbo MB,Mallia P,Sidney J,Peters B,Kon OM,Sette A,Johnston SL,Openshaw PJ,Chiu C

Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry

Mon Dec 21 00:00:00 EST 2015

Liposomal Glutathione Supplementation Restores TH1 Cytokine Response to Mycobacterium tuberculosis Infection in HIV-Infected Individuals.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research

Ly J,Lagman M,Saing T,Singh MK,Tudela EV,Morris D,Anderson J,Daliva J,Ochoa C,Patel N,Pearce D,Venketaraman V

Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2015

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2015

CD4+CD25(high), CD8+CD28- cells and thyroid autoantibodies in breast cancer patients.

Central-European journal of immunology

Biylgi O,Karagöz B,Türken O,Gültepe M,Özgün A,Tunçel T,Emirzeoğlu L,Çelik S,Müftüoğlu T,Gökhan Kandemir E

Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry

Thu Jul 09 00:00:00 EDT 2015

Accumulation of 4-1BBL+ B cells in the elderly induces the generation of granzyme-B+ CD8+ T cells with potential antitumor activity.

Blood

Lee-Chang C,Bodogai M,Moritoh K,Olkhanud PB,Chan AC,Croft M,Mattison JA,Holst PJ,Gress RE,Ferrucci L,Hakim F,Biragyn A

25-0088 was used in Flow cytometry/Cell sorting to propose that 4BL cells and the 4-1BBL signaling pathway are useful targets for improved effectiveness of natural antitumor defenses and therapeutic immune manipulations in the elderly.

Thu Aug 28 00:00:00 EDT 2014

Immune response to JC virus T antigen in patients with and without colorectal neoplasia.

Gut microbes

Butcher LD,Garcia M,Arnold M,Ueno H,Goel A,Boland CR

Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry

Tue Jul 01 00:00:00 EDT 2014

Galectin-9 activates and expands human T-helper 1 cells.

PloS one

Gooden MJ,Wiersma VR,Samplonius DF,Gerssen J,van Ginkel RJ,Nijman HW,Hirashima M,Niki T,Eggleton P,Helfrich W,Bremer E

25-0088 was used in Flow cytometry/Cell sorting to demonstrate that galectin-9 activates resting T-cells and promotes their transition to a T-helper 1 cell phenotype.

Fri Jan 10 00:00:00 EST 2014

Ex vivo analysis of resident hepatic pro-inflammatory CD1d-reactive T cells and hepatocyte surface CD1d expression in hepatitis C.

Journal of viral hepatitis

Yanagisawa K,Yue S,van der Vliet HJ,Wang R,Alatrakchi N,Golden-Mason L,Schuppan D,Koziel MJ,Rosen HR,Exley MA

Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2013

Upregulation of programmed death-1 on T cells and programmed death ligand-1 on monocytes in septic shock patients.

Critical care (London, England)

Zhang Y,Li J,Lou J,Zhou Y,Bo L,Zhu J,Zhu K,Wan X,Cai Z,Deng X

Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry

Wed Dec 07 00:00:00 EST 2011

Purification and direct transformation of epithelial progenitor cells from primary human prostate.

Nature protocols

Goldstein AS,Drake JM,Burnes DL,Finley DS,Zhang H,Reiter RE,Huang J,Witte ON

25-0088 was used in Flow cytometry/Cell sorting to describe a system to directly transform freshly isolated primary human prostate epithelial cells without previous culture or immortalisation.

Sun May 01 00:00:00 EDT 2011

B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity.

Retrovirology

Brudek T,Christensen T,Aagaard L,Petersen T,Hansen HJ,Møller-Larsen A

25-0088 was used in Flow cytometry/Cell sorting to study the increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity, in B cells and monocytes from patients with active multiple sclerosis.

Mon Nov 16 00:00:00 EST 2009

Sepsis-induced human lymphocyte apoptosis and cytokine production in "humanized" mice.

Journal of leukocyte biology

Unsinger J,McDonough JS,Shultz LD,Ferguson TA,Hotchkiss RS

Published figure using CD8a monoclonal antibody (Product # 25-0088-42) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

UniProt ID: (Human) P01732, (Human) P10966

Entrez Gene ID: (Human) 925, (Human) 926

Molecular Function: defense/immunity protein immunoglobulin receptor superfamily