Invitrogen

CDK5RAP3 Polyclonal Antibody

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CDK5RAP3 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of CDK5RAP3 in SHSY5Y cells using a CDK5RAP3 polyclonal antibody (Product #PA5-116477). Formalin fixed cells, as seen in green, were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody ( 1:100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI (blue).

CDK5RAP3 Antibody in Western Blot (WB) — Western blot analysis of CDK5RAP3 in MCF-7 cell lysate using a CDK5RAP3 polyclonal antibody (Product #PA5-116477). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

CDK5RAP3 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of CDK5RAP3 in SHSY5Y cells using a CDK5RAP3 polyclonal antibody (Product#PA5-116477). The cells were fixed, permeabilize, and stained with the primary antibody (1:100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1:500 dilution for 30 minutes. The control was not incubated with the primary antibody (black).

Product Details

PA5-116477

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein within Human CDK5RAP3 aa 296-506 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4, with 0.2% BSA, 50% glycerol
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Positive controls: MCF-7 cell lysates, LOVO, PC-3M, SHSY5Y.

Target Information

This gene encodes a protein that has been reported to function in signaling pathways governing transcriptional regulation and cell cycle progression. It may play a role in tumorigenesis and metastasis. A pseudogene of this gene is located on the long arm of chromosome 20. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, May 2013].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CDK5 activator-binding protein C53; CDK5 regulatory subunit associated protein IC53-2; CDK5 regulatory subunit-associated protein 3; IC53-2; ischemic heart CDK5 activator-binding protein C53; LXXLL/leucine-zipper-containing ARF-binding protein; LXXLL/leucine-zipper-containing ARFbinding protein; Protein HSF-27

Gene Aliases: C53; CDK5RAP3; HSF-27; IC53; LZAP; MST016; MSTP016; OK/SW-cl.114; PP1553

UniProt ID: (Human) Q96JB5

Entrez Gene ID: (Human) 80279

Function(s)