Invitrogen

CDK5RAP3 Polyclonal Antibody

View all (14) CDK5RAP3 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (5)

CDK5RAP3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

CDK5RAP3 Antibody (PA5-116477) in ICC/IF

Immunocytochemical analysis of CDK5RAP3 in SHSY5Y cells using a CDK5RAP3 polyclonal antibody (Product #PA5-116477). Formalin fixed cells, as seen in green, were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody ( 1:100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI (blue).

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CDK5RAP3 Antibody in Western Blot (WB) — Western blot analysis of CDK5RAP3 in MCF-7 cell lysate using a CDK5RAP3 polyclonal antibody (Product #PA5-116477). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

CDK5RAP3 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of CDK5RAP3 in SHSY5Y cells using a CDK5RAP3 polyclonal antibody (Product#PA5-116477). The cells were fixed, permeabilize, and stained with the primary antibody (1:100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1:500 dilution for 30 minutes. The control was not incubated with the primary antibody (black).

Product Details

PA5-116477

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

Immunocytochemistry (ICC/IF)

1:50-1:200

Flow Cytometry (Flow)

1:50-1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein within Human CDK5RAP3 aa 296-506

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2901109

Product Specific Information

Positive controls: MCF-7 cell lysates, LOVO, PC-3M, SHSY5Y.

Target Information

This gene encodes a protein that has been reported to function in signaling pathways governing transcriptional regulation and cell cycle progression. It may play a role in tumorigenesis and metastasis. A pseudogene of this gene is located on the long arm of chromosome 20. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, May 2013].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CDK5 activator-binding protein C53; CDK5 regulatory subunit associated protein IC53-2; CDK5 regulatory subunit-associated protein 3; IC53-2; ischemic heart CDK5 activator-binding protein C53; LXXLL/leucine-zipper-containing ARF-binding protein; LXXLL/leucine-zipper-containing ARFbinding protein; Protein HSF-27

Gene Aliases: C53; CDK5RAP3; HSF-27; IC53; LZAP; MST016; MSTP016; OK/SW-cl.114; PP1553

UniProt ID: (Human) Q9H8G0

Entrez Gene ID: (Human) 80279

Function(s)

regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of protein phosphorylation mitotic G2 DNA damage checkpoint brain development cell proliferation regulation of phosphatase activity apoptotic nuclear changes endoplasmic reticulum unfolded protein response positive regulation of protein ubiquitination negative regulation of NF-kappaB transcription factor activity negative regulation of MAP kinase activity negative regulation of protein kinase activity by regulation of protein phosphorylation mitotic G2/M transition checkpoint regulation of neuron differentiation positive regulation of transcription from RNA polymerase II promoter protein ufmylation negative regulation of protein serine/threonine kinase activity positive regulation of protein localization to nucleus positive regulation of signal transduction by p53 class mediator negative regulation of cellular protein catabolic process positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process

Process(es)

protein binding protein kinase binding cyclin binding protein complex binding ubiquitin-like protein ligase binding mitogen-activated protein kinase binding NF-kappaB binding MDM2/MDM4 family protein binding