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CD163 Monoclonal Antibody (GHI/61), CoraLite® Plus 594
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FIGURE: 1 / 1
CD163 Antibody (CL594-65169) in Flow
1x10^6 human PBMCs were surface stained with CL488 Anti-Human CD14 (CL488-65246) and 5 µL CoraLite594 Anti-Human CD163 (CL594-65169, Clone: GHI/61) or CoraLite594 Mouse IgG1 Isotype Control (CL594-65124, Clone: MOPC-21). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.
Product Details
CL594-65169
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
GHI/61
Immunogen
Glycoprotein preparation from hairy cell leukemia spleen
Conjugate
Excitation/Emission Max
588/604 nm
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS with 0.5% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Wet ice
Target Information
CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein, a member of the scavenger receptor cysteine-rich superfamily, and a receptor for the hemoglobin-haptoglobin complex. CD163 protects tissues from free hemoglobin-mediated oxidative damage, and may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. Specifically, CD163 is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan's cells. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant. CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, and exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. Further, CD163 also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD163; Hemoglobin scavenger receptor; M130; macrophage-associated antigen; Scavenger receptor cysteine-rich type 1 protein M130; Soluble sCD163
Gene Aliases: CD163; M130; MM130; SCARI1
UniProt ID: (Human) Q86VB7
Entrez Gene ID: (Human) 9332
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