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CLIC1 Antibody (PA5-119713) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of CLIC1 in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with CLIC1 Polyclonal Antibody (Product # PA5-119713) at a dilution of 1:200 for at least 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI (blue).
Product Details
PA5-119713
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within Human CLIC1 aa 1-200
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2913286
Product Specific Information
Positive Control: Mouse kidney tissue lysates, A431, MG-63, SKOV-3, rat large intestine tissue, human kidney tissue, human placenta tissue, mouse colon tissue.
Subcellular Location: Cell membrane, Cytoplasm, Membrane, Nucleus.
Target Information
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Chloride channel ABP; Chloride intracellular channel protein 1; chloride intracellular channel protein 1 {ECO:0000250|UniProtKB:O00299, ECO:0000312|EMBL:CAE83985.1}; clic1 {ECO:0000312|EMBL:CAE83985.1, ECO:0000312|RGD:1303043}; DADB-110M10.1; FLJ94671; Glutaredoxin-like oxidoreductase CLIC1; Glutathione-dependent dehydroascorbate reductase CLIC1; hRNCC; NCC27; Nuclear chloride ion channel 27; nuclear chloride ion channel protein; p64CLCP; Regulatory nuclear chloride ion channel protein; RNCC protein
Gene Aliases: Clcp; CLIC1; G6; NCC27
UniProt ID: (Human) O00299, (Mouse) Q9Z1Q5, (Rat) Q6MG61
Entrez Gene ID: (Human) 1192, (Mouse) 114584, (Rat) 406864
glutathione metabolic process
chloride transport
signal transduction
regulation of ion transmembrane transport
positive regulation of osteoblast differentiation
regulation of cell cycle
regulation of mitochondrial membrane potential
platelet aggregation
chloride transmembrane transport
transport
ion transport
regulation of anion transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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