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FIGURE: 1 / 8
CaMKI Antibody (PA1-543) in ICC/IF
Immunofluorescence analysis of CaM Kinase I was performed using 70% confluent log phase U-87MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CaM Kinase I Rabbit Polyclonal Antibody (Product # PA1-543) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjuga... View More
Product Details
PA1-543
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: A(1) V E G P R W K Q A E D I R D I Y D(18)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2070105
Product Specific Information
PA1-543 detects calciµm/calmodulin-dependent kinase I (CaMKI) from rat and mouse brain extract.
PA1-543 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~43 kDa protein representing CaMKI from rat and mouse brain extract.
PA1-543 immunizing peptide corresponds to amino acid residues 1-18 from human CaMKI protein. PA1-543 immunizing peptide (Cat. # PEP-134) is available for use in neutralization and control experiments.
Target Information
CAMK1 (CaMK1) is a serine/threonine kinase that is a member of the calcium/calmodulin-dependent protein kinase family. It is involved in cell differentiation, nervous system development, and signal transduction. CAMK1 is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: calcium/calmodulin-dependent protein kinase I; Calcium/calmodulin-dependent protein kinase type 1; CaM kinase I; CaM kinase I alpha; CaM-KI; CAMK1-PEN; CaMKI-alpha; G alpha interacting protein; MGC120317; MGC120318; regulator of G-protein signalling 19
Gene Aliases: AI505105; CAMK1; CAMKI; CaMKIalpha; D6Ertd263e; Gaip
UniProt ID: (Human) Q14012, (Rat) Q63450, (Mouse) Q91YS8
Entrez Gene ID: (Human) 8536, (Rat) 171503, (Mouse) 52163
calmodulin-dependent protein kinase activity
protein binding
calmodulin binding
ATP binding
nucleotide binding
catalytic activity
protein kinase activity
protein serine/threonine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein phosphorylation
nucleocytoplasmic transport
cell cycle
signal transduction
positive regulation of neuron projection development
negative regulation of protein binding
regulation of protein localization
positive regulation of peptidyl-serine phosphorylation
regulation of protein binding
positive regulation of transcription from RNA polymerase II promoter
positive regulation of protein export from nucleus
regulation of muscle cell differentiation
positive regulation of muscle cell differentiation
positive regulation of synapse structural plasticity
positive regulation of syncytium formation by plasma membrane fusion
positive regulation of dendritic spine development
positive regulation of protein serine/threonine kinase activity
positive regulation of protein acetylation
regulation of histone H3-K9 acetylation
multicellular organism development
nervous system development
metabolic process
phosphorylation
cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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