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Invitrogen
DFNA5 Polyclonal Antibody
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Product Details
PA5-82429
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human DFNA5. Recombinant protein control fragment (Product #RP-102091).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Amount
10 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: VLFDDELLMV LEPVCDDLVS GLSPTVAVLG ELKPRQQQDL VAFLQLVGCS LQGGCPGPED AGSKQLFMTA YFLVSALAEM PDSAAALLGT CCKLQIIPTL CHLLRALSDD GVSDLEDPTL TPLKDTERFG IVQRLFASAD ISLERLKSSV
Highest antigen sequence indentity to the following orthologs: Rat - 67%, Mouse - 66%.
Target Information
GSDME (Gasdermin E) is a member of the gasdermin family of pore-forming proteins that regulate programmed cell death and inflammatory signaling. Encoded by the GSDME gene, the full-length protein contains an N-terminal pore-forming domain and a C-terminal autoinhibitory domain. Under apoptotic conditions, activation of caspase-3 leads to proteolytic cleavage of GSDME, releasing the N-terminal fragment, which oligomerizes and inserts into the plasma membrane to form pores. This process can convert non-lytic apoptosis into a lytic, inflammatory form of cell death resembling pyroptosis, resulting in membrane rupture and release of intracellular contents. GSDME has been implicated in cancer biology, chemotherapy response, and tissue injury, and was originally identified in association with autosomal dominant hearing loss (historically referred to as DFNA5). Detection of cleaved GSDME is commonly used as a marker of caspase-3-mediated pore formation and inflammatory cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DFNA5; DFNA5, deafness associated tumor suppressor; expression inversely correlated with estrogen receptor expression in breast cancer; Gasdermin E; Gasdermin-E; ICERE-1; Inversely correlated with estrogen receptor expression 1; Non-syndromic hearing impairment protein 5; nonsyndromic hearing impairment protein; unnamed protein product
Gene Aliases: DFNA5; GSDME; ICERE-1; ICERE1
UniProt ID: (Human) O60443
Entrez Gene ID: (Human) 1687
positive regulation of immune response to tumor cell
sensory perception of sound
negative regulation of cell proliferation
programmed cell death
auditory receptor cell differentiation
positive regulation of MAPK cascade
transmembrane transport
pyroptosis
cellular response to tumor necrosis factor
cellular response to virus
granzyme-mediated programmed cell death signaling pathway
pyroptotic cell death
positive regulation of intrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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