Immunofluorescence analysis of DISC1 was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with DISC1 Rabbit Polyclonal Antibody (406900) at 1ug/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing Nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from an internal region of the mouse DISC1 (disrupted in schizophrenia 1) isoforms 1 and 2, which differ from rat by one amino acid|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunocytochemistry (ICC)||1 µg/ml|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
DISC1 (Disrupted in Schizophrenia 1) is a multifunctional protein that interacts with multiple proteins of the centrosome and cytoskeletal system, and is involved in neuronal functions that are dependent upon proper cytoskeletal regulation, including neuronal migration, neurite architecture and intracellular transport. Regions of the brain which express DISC1 include the hippocampus, lateral septum, amygdala, cerebral cortex, cerebellum and paraventricular hypothalamus, and loss of DISC1 function results in differences in structural organization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
An inherited duplication at the gene p21 Protein-Activated Kinase 7 (PAK7) is a risk factor for psychosis.
40-6900 was used in immunohistochemistry - free floating and western blot to identify rare, highly penetrant risk mutations that contribute to schizophrenia.
|Morris DW,Pearson RD,Cormican P,Kenny EM,O'Dushlaine CT,Perreault LP,Giannoulatou E,Tropea D,Maher BS,Wormley B,Kelleher E,Fahey C,Molinos I,Bellini S,Pirinen M,Strange A,Freeman C,Thiselton DL,Elves RL,Regan R,Ennis S,Dinan TG,McDonald C,Murphy KC,O'Callaghan E,Waddington JL,Walsh D,O'Donovan M,Grozeva D,Craddock N,Stone J,Scolnick E,Purcell S,Sklar P,Coe B,Eichler EE,Ophoff R,Buizer J,Szatkiewicz J,Hultman C,Sullivan P,Gurling H,Mcquillin A,St Clair D,Rees E,Kirov G,Walters J,Blackwood D,Johnstone M,Donohoe G,O'Neill FA,Kendler KS,Gill M,Riley BP,Spencer CC,Corvin A||Human molecular genetics (23:3316)||2014|
|DISC1 regulates primary cilia that display specific dopamine receptors.||Marley A,von Zastrow M||PloS one (5:null)||2010|
|Mouse||Not Cited||Disc1 regulates granule cell migration in the developing hippocampus.||Meyer KD,Morris JA||Human molecular genetics (18:3286)||2009|