Invitrogen

DST Polyclonal Antibody

Product Image Gallery

DST Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of DST in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and DST polyclonal antibody (Product # PA5-106906) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

DST Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded DST in human lung tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with DST polyclonal antibody (Product # PA5-106906) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

DST Antibody in Western Blot (WB) — Western blot analysis of DST in various samples (Lane 1: 3T3, treated with blocking peptide, Lane 2: 3T3, Lane 3: Colo205). Samples were incubated with DST polyclonal antibody (Product # PA5-106906).

Product Details

PA5-106906

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human DST, corresponding to a region within C-terminal amino acids. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2854570

Target Information

As basal cells of stratified squamous epithelia begin to migrate in response to wound healing, they lose their cell-substrate adhesion junctions, the hemidesmosomes. The hemidesmosome is an adhesion structure of the epidermal-dermal junction in keratinocytes. When keratinocytes migrate laterally or upward to differentiate they must control the formation and disintegration of the hemidesmosomes. The bullous pemphigoid antigen BPAG1 is a hemidesmosomal protein of the cutaneous basement membrane zone. The primary sequence deduced from full-length human cDNAs predicts that this molecule consists of a central rod region and flanking globular domains. A neuronal isoform, BPAG1n3 is the result of differential splicing of BPAG1. BPAG1n3 is distinguished by its initial 32 amino acid residues and by the absence of the amino-terminal half of the actin-binding domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: 230 kDa bullous pemphigoid antigen; 230/240 kDa bullous pemphigoid antigen; BPA; Bullous pemphigoid antigen 1; bullous pemphigoid antigen 1, isoform 5; bullous pemphigoid antigen 1, isoforms 1/2/3/4; bullous pemphigoid antigen 1, isoforms 6/7; Dystonia musculorum protein; Dystonin; Hemidesmosomal plaque protein; Microtubule actin cross-linking factor 2; trabeculin-beta

Gene Aliases: 2310001O04Rik; A830042E19Rik; ah; athetoid; AW554249; BP230; BP240; BPA; Bpag; BPAG1; BPAG1-n; CATX-15; CATX15; D6S1101; DMH; DST; DT; EBSB2; HSAN6; KIAA0728; MACF2; mKIAA0728; nmf203; nmf339

UniProt ID: (Human) Q03001, (Mouse) Q91ZU6

Entrez Gene ID: (Human) 667, (Mouse) 13518, (Rat) 316313

Molecular Function: intermediate filament binding protein cytoskeletal protein intermediate filament