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DUT Polyclonal Antibody

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DUT Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HEK-293 cells using 13740-1-AP (DUT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

DUT Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart tissue slide using 13740-1-AP ( DUT Antibody) at dilution of 1:200 (under 40x lens).

DUT Antibody in Western Blot (WB) — Human kidney tissue were subjected to SDS PAGE followed by western blot with 13740-1-AP (DUT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Product Details

13740-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2400	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DUT Fusion Protein Ag4677 (89-252 aa encoded by BC033645) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.39 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MPCSEETPA ISPSKRARPA EVGGMQLRFA RLSEHATAPT RGSARAAGYD LYSAYDYTIP PMEKAVVKTD IQIALPSGCY GRVAPRSGLA AKHFIDVGAG VIDEDYRGNV GVVLFNFGKE KFEVKKGDRI AQLICERIFY PEIEEVQALD DTERGSGGFG STGKN (89-252 aa encoded by B C033645)

Target Information

This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ATP-Linked Chimeric Nucleotide as a Specific Luminescence Reporter of Deoxyuridine Triphosphatase.

Bioconjugate chemistry

Ji D,Kietrys AM,Lee Y,Kool ET

Wed May 16 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial; dUTP diphosphatase; dUTP nucleotidohydrolase; dUTP pyrophosphatase; dUTPase; FLJ20622

Gene Aliases: DUT; dUTPase

UniProt ID: (Human) P33316

Entrez Gene ID: (Human) 1854

Molecular Function: hydrolase phosphatase metabolite interconversion enzyme

Function(s)

nucleobase-containing compound metabolic process dUMP biosynthetic process DNA replication dUTP catabolic process pyrimidine nucleoside biosynthetic process

Process(es)

magnesium ion binding dUTP diphosphatase activity protein binding poly(A) RNA binding

DUT Polyclonal Antibody

Product Details

13740-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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