Proteintech

E-cadherin Polyclonal Antibody

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E-cadherin Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ( 4% PFA) fixed human breast cancer tissue using 20874-1-AP (E-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

E-cadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 20874-1-AP (E-cadherin Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

E-cadherin Antibody in Western Blot (WB) — Mouse testis tissue were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

E-cadherin Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with .2ug E-cadherin antibody (20874-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

E-cadherin Antibody in Immunoprecipitation (IP) — IP result of anti-E-cadherin (IP:20874-1-AP, 3ug; Detection:20874-1-AP 1:1000) with A431 cells lysate 3000ug.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunocytochemistry (ICC)

1:20-1:200

Immunofluorescence (IF)

1:20-1:200

View 46 publications 46 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2000

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:1000-1:4000

View 178 publications 178 publications

Immunohistochemistry (IHC)

View 47 publications 47 publications

Miscellaneous PubMed (Misc)

View 16 publications 16 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bovine, Human, Mouse, Pig, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E-cadherin Fusion Protein Ag14973

Conjugate

Unconjugated

Form

Liquid

Concentration

0.34 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This polyclonal antibody recognizes 120 kDa intact E-cadherin and its 80 kDa trypsin-cleaved fragment.

Target Information

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ARC-1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; cell-CAM 120/80; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3; E-Cadherin; E-cadherin 1; E-Cadherin phospho; Ecadherin; Epithelial cadherin; phospho cadherin 1; phospho E-Cadherin; phospho LCAM; phospho S838; phospho UVO; S840; S840 E-Cadherin; Ser840; Ser840 E-Cadherin; Uvomorulin

Gene Aliases: AA960649; Arc-1; CD324; CDH1; CDHE; E-cad; ECAD; L-CAM; LCAM; Um; UVO

UniProt ID: (Human) P12830, (Mouse) P09803, (Rat) Q9R0T4

Entrez Gene ID: (Human) 999, (Mouse) 12550, (Rat) 83502

Molecular Function: cadherin