|Tested species reactivity||Bovine, Human, Non-human primate|
|Published species reactivity||Bovine|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Bacterial expressed full length GCAP-1.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-724 detects GCAP-1 from human, non-human primate and bovine samples. This antibody is specific for GCAP-1 and does not react with other isotypes.
MA1-724 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~23 kDa protein representing GCAP-1 protein on bovine rod outer segment membranes.
The MA1-724 immunogen is bacterial expressed full length GCAP-1.
Guanylate cyclase-activating proteins (GCAPs) are calcium binding proteins that belong to the calmodulin superfamily. GCAPs without calcium are responsible for activation of photoreceptor guanylate cyclase during light adaptation. Studies have shown that the addition or subtraction of Ca2+ results in major conformational changes and the activation/deactivation of GCAP-1 and GCAP-2.
It has been demonstrated that both GCAP-1 and 2 act on guanylate cyclase similarly and have approximately 50% homology between the two forms. GCAP-1 (GenBank AF172707) is predominantly localized in the photoreceptor outer segments, while GCAP-2 is found in retina samples.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Conformational changes in guanylyl cyclase-activating protein 1 (GCAP1) and its tryptophan mutants as a function of calcium concentration.
MA1-724 was used in immunoprecipitation to investigate the important roles of Trp residues in the function of GCAP1 and its conformational changes mediated by calcium binding
|Sokal I,Otto-Bruc AE,Surgucheva I,Verlinde CL,Wang CK,Baehr W,Palczewski K||The Journal of biological chemistry (274:19829)||1999|
Functional reconstitution of photoreceptor guanylate cyclase with native and mutant forms of guanylate cyclase-activating protein 1.
MA1-724 was used in immunoprecipitation to investigate the mechanism for the restoration of the functions of retGC1 by GCAP1
|Otto-Bruc A,Buczylko J,Surgucheva I,Subbaraya I,Rudnicka-Nawrot M,Crabb JW,Arendt A,Hargrave PA,Baehr W,Palczewski K||Biochemistry (36:4295)||1997|
Phosphorylation of photolyzed rhodopsin is calcium-insensitive in retina permeabilized by alpha-toxin.
MA1-724 was used in western blot to investigate the influence of calcium on rhodopsin phosphorylation in retina
|Otto-Bruc AE,Fariss RN,Van Hooser JP,Palczewski K||Proceedings of the National Academy of Sciences of the United States of America (95:15014)||1998|
Localization of guanylate cyclase-activating protein 2 in mammalian retinas.
MA1-724 was used in western blot to investigate the distribution of guanylate cyclase-activating protein 2 in human, monkey and bovine retinas
|Otto-Bruc A,Fariss RN,Haeseleer F,Huang J,Buczy¿ko J,Surgucheva I,Baehr W,Milam AH,Palczewski K||Proceedings of the National Academy of Sciences of the United States of America (94:4727)||1997|
Molecular cloning and characterization of retinal photoreceptor guanylyl cyclase-activating protein.
MA1-724 was used in blocking/activating experiment to clone guanylyl cyclase-activating protein and to investigate its functional properties
|Palczewski K,Subbaraya I,Gorczyca WA,Helekar BS,Ruiz CC,Ohguro H,Huang J,Zhao X,Crabb JW,Johnson RS||Neuron (13:395)||1994|