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GCN1L1 Antibody (A301-843A) in WB
Detection of mouse and rat GCN1L1 by western blot. Samples: Whole cell lysate (50 µg) from NIH3T3, TCMK-1, 4T1, and C6 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GCN1L1 antibody A301-843A (lot A301-843A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A301-843A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 2621 and 2671 of human general control of amino-acid synthesis 1-like 1.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Acts as a positive activator of the EIF2AK4/GCN2 protein kinase activity in response to amino acid starvation. Forms a complex with EIF2AK4/GCN2 on translating ribosomes; during this process, GCN1 seems to act as a chaperone to facilitate delivery of uncharged tRNAs that enter the A site of ribosomes to the tRNA-binding domain of EIF2AK4/GCN2, and hence stimulating EIF2AK4/GCN2 kinase activity. Participates in the repression of global protein synthesis and in gene-specific mRNA translation activation, such as the transcriptional activator ATF4, by promoting the EIF2AK4/GCN2-mediated phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52', and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GCN1 (general control of amino-acid synthesis 1, yeast)-like 1; GCN1 eIF-2-alpha kinase activator homolog; GCN1 eIF2 alpha kinase activator homolog; GCN1 general control of amino-acid synthesis 1-like 1; GCN1-like protein 1; General control of amino-acid synthesis 1-like protein 1; HsGCN1; peroxisome proliferator activated receptor interacting complex protein; Stalled ribosome sensor GCN1; Translational activator GCN1
Gene Aliases: 4932409G22; AL022764; G431004K08Rik; GCN1; GCN1L; GCN1L1; KIAA0219; mKIAA0219; PRIC295
UniProt ID: (Human) Q92616, (Mouse) E9PVA8
Entrez Gene ID: (Human) 10985, (Mouse) 231659, (Rat) 690632
translation
regulation of translation
positive regulation of kinase activity
cellular response to amino acid starvation
positive regulation of transcription from RNA polymerase II promoter in response to stress
regulation of protein kinase activity
cell-cell adhesion
cellular response to leucine starvation
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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