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Invitrogen
Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor™ 680
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FIGURE: 1 / 6
Rabbit IgG (Heavy Chain) Secondary Antibody (A27042) in WB
Expression of endogenous IRAK1 (~80KDa) was detected using an Anti-IRAK1 Recombinant Rabbit Polyclonal Antibody (Product # 710015, 0.5 µg/mL) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor 680 Conjugate (Product # A27042) (0.4 µg/mL final concentration, 1:2500 dilution). Western blot analysis was performed on whole cell extracts (20 µg lysate) of various cell types using... View More
Product Details
A27042
Applications
Tested Dilution
Western Blot (WB)
0.05-2 µg/mL
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Goat
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Secondary Antibody
Immunogen
Recombinant full-length protein
Conjugate
Excitation/Emission Max
681/704 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
1 mg
Purification
purified
Storage buffer
PBS, pH 7.2
Contains
5mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Target
IgG (Heavy chain)
Antibody Form
Whole Antibody
Product Specific Information
The sensitivity and specificity of each lot is confirmed using Western blot.
Minimal cross-reactivity with rabbit, mouse, rat, human, bovine, guinea pig and donkey IgG is observed.
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and more lot-to-lot consistency.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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