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          • Primary Antibodies ›
          • H4K5ac Antibodies
          We recommend SNAP-ChIP™ spike-in validated H4K5ac antibody for ChIP.

          Invitrogen

          H4K5ac Polyclonal Antibody

          View all (16) H4K5ac antibodies

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          Cite H4K5ac Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (5)
          H4K5ac Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          H4K5ac Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          H4K5ac Antibody (PA5-90308) in IHC (P)

          Immunohistochemistry analysis of H4K5ac in paraffin-embedded mouse kidney. Samples were incubated with H4K5ac Polyclonal antibody (Product # PA5-90308) using a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          H4K5ac Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          H4K5ac Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          H4K5ac Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          H4K5ac Antibody in Western Blot (WB)
          H4K5ac Antibody in ChIP Assay (ChIP)
          H4K5ac Polyclonal Antibody

          Product Details

          PA5-90308

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          ELISA (ELISA)

          1 µg/mL
          -

          ChIP assay (ChIP)

          5 µg antibody for 5 µg-10 µg of Chromatin
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H4 (NP_0010292491)
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.14 mg/mL

          Amount

          114 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.05% ProClin 300

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2806015

          Product Specific Information

          Positive Samples: HeLa, C6; Cellular Location: Chromosome, Nucleus

          Immunogen sequence: MSGRGKGGKG LGK

          Target Information

          Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: acetyl Histone H4; Acetyl-H4 K5; Acetyl-H4 Lys5; Acetyl-Histone H4 K5; Acetyl-Histone H4 Lys5; H4 histone family, member A; H4 histone family, member E; H4 histone family, member I; H4 histone family, member J; H4 histone family, member M; H4 histone family, member N; H4 histone, family 2; H4 K5ac; H4K5; H4Lys5ac; HIST1H4; HIST1H4F; HIST2H4; histone 1, H4a; histone 1, H4b; histone 1, H4e; histone 1, H4i; histone 1, H4j; histone 2, H4; histone 2, H4a; Histone 4; Histone 4 family, member M; histone 4, H4; histone cluster 1 H4 family member a; histone cluster 1 H4 family member b; histone cluster 1 H4 family member e; histone cluster 1 H4 family member i; histone cluster 1 H4 family member j; histone cluster 1, H4a; histone cluster 1, H4b; histone cluster 1, H4e; histone cluster 1, H4i; histone cluster 1, H4j; histone cluster 2 H4 family member a; histone cluster 2, H4a; Histone Cluster 4; histone cluster 4 H4; histone family member; Histone H4; Histone H4 acetyl; Histone H4A; Histone H4K5; Histone H4K5ac; histone IV, family 2; unnamed protein product

          View more View less

          Gene Aliases: dJ160A22.2; FO108; H4; H4-12; H4-16; H4-53; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4f16; H4F2; H4F2iv; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4M; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; Hist1h4m; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4; TEBIVANED2; TEBIVANED3; TEBIVANED4; TEVANED2; TEVANED3; TEVANED4; X04652

          View more View less

          UniProt ID: (Human) P62805, (Mouse) P62806, (Mouse) A4QMY0

          View more View less

          Entrez Gene ID: (Human) 8359, (Human) 8363, (Human) 8370, (Human) 121504, (Human) 8366, (Human) 8367, (Human) 8294, (Mouse) 326619, (Mouse) 326620, (Mouse) 319158, (Mouse) 97122

          View more View less

          Function(s)
          DNA binding RNA binding protein binding structural constituent of chromatin protein heterodimerization activity histone binding poly(A) RNA binding histone
          Process(es)
          chromatin organization nucleosome assembly telomere organization negative regulation of megakaryocyte differentiation protein localization to CENP-A containing chromatin DNA replication-dependent nucleosome assembly DNA replication-independent nucleosome assembly protein heterotetramerization
          It has to be done as per old AB suggested Products section.

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