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FIGURE: 1 / 4
H4K5ac Antibody (712906) in ICC/IF
For immunofluorescence analysis, HeLa cells were fixed and permeabilized with 0.5% Triton X-100 for detection of endogenous H4K5ac using Anti-H4K5ac Recombinant Rabbit Superclonal™ Antibody (Product # 712906, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of H4K5ac protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifad... View More
Product Details
712906
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
41HCLC
Immunogen
Peptide corresponding to human H4K5ac [aa1-aa9]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. This antibody is predicted to react with Mouse, Rat, Bovine. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acetyl Histone H4; Acetyl-H4 K5; Acetyl-H4 Lys5; Acetyl-Histone H4 K5; Acetyl-Histone H4 Lys5; H4 histone family, member A; H4 histone family, member E; H4 histone family, member I; H4 histone family, member J; H4 histone family, member M; H4 histone family, member N; H4 histone, family 2; H4 K5ac; H4K5; H4Lys5ac; HIST1H4; HIST1H4F; HIST2H4; histone 1, H4a; histone 1, H4b; histone 1, H4e; histone 1, H4i; histone 1, H4j; histone 2, H4a; Histone 4; Histone 4 family, member M; histone 4, H4; histone cluster 1 H4 family member a; histone cluster 1 H4 family member b; histone cluster 1 H4 family member e; histone cluster 1 H4 family member i; histone cluster 1 H4 family member j; histone cluster 1, H4a; histone cluster 1, H4b; histone cluster 1, H4e; histone cluster 1, H4i; histone cluster 1, H4j; histone cluster 2 H4 family member a; histone cluster 2, H4a; Histone Cluster 4; histone cluster 4 H4; histone family member; Histone H4; Histone H4 acetyl; Histone H4A; Histone H4K5; Histone H4K5ac; histone IV, family 2; unnamed protein product
Gene Aliases: dJ160A22.2; FO108; H4; H4-16; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4F2; H4F2iv; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4M; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4; TEBIVANED2; TEBIVANED3; TEBIVANED4; TEVANED2; TEVANED3; TEVANED4
UniProt ID: (Human) P62805
Entrez Gene ID: (Human) 8359, (Human) 8363, (Human) 8370, (Human) 121504, (Human) 8366, (Human) 8367, (Human) 8294
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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