Invitrogen

HLA-DOA Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (5) HLA-DOA antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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HLA-DOA Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of HLA-DOA in human spleen and liver tissues using HLA-DOA Polyclonal Antibody (Product # PA5-60904). Corresponding HLA-DOA RNA-seq data are presented for the same tissues.

HLA-DOA Antibody in Western Blot (WB) — Western blot analysis of HLA-DOA in control (vector only transfected HEK293T lysate) and HLA-DOA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a HLA-DOA Polyclonal Antibody (Product # PA5-60904).

HLA-DOA Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of HLA-DOA demonstrates antibody specificity. Immunohistochemical analysis of HLA-DOA using anti-HLA-DOA Polyclonal Antibody (Product # PA5-60904), shows significant staining of HLA-DOA in human spleen and shows minimal or weak staining in human liver tissues. The relative expression levels of HLA-DOA within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-60904

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:200-1:500	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human HLA-DOA View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.4 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2642460

Product Specific Information

Immunogen sequence: GDFARFDPQG GLAGIAAIKA HLDILVERSN RSRAINVPPR VTVLPKSRVE

Highest antigen sequence identity to the following orthologs: Mouse - 76%, Rat - 70%.

Target Information

Reacts with HLA-DO, a heterodimer formed by DNalpha and DObeta subunits in B cells. DNalpha and DObeta are the products of the non-classical class II genes, HLA-DNA and HLA-DOB, respectively. DO forms tight complexes with DM in the endoplasmic reticulum and is thereby sorted to lysosomal vesicles during antigen processing and presentation. It is tightly associated with DM and it is selectively expressed on antigen-presenting cells, such as B cells, dendritic cells and thymic epithelial cells. DO can enhance the efficiency of peptide loading and has been found to stabilize DM at low pH, preserving its chaperon activity. DO-DM complexes are more efficient than DM in protecting empty DR molecules. Reports describe DO as a co-chaperone of DM.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HLA class II histocompatibility antigen, DO alpha chain; HLA-D0-alpha; lymphocyte antigen; major histocompatibility complex, class II, DN alpha; MHC class II antigen DOA; MHC DN-alpha; MHC DZ alpha

Gene Aliases: HLA-DNA; HLA-DOA; HLA-DZA; HLADZ

UniProt ID: (Human) P06340

Entrez Gene ID: (Human) 3111

Molecular Function: major histocompatibility complex protein

Function(s)

negative regulation of antigen processing and presentation of peptide antigen via MHC class II immune response signal transduction antigen processing and presentation of exogenous peptide antigen via MHC class II regulation of T cell differentiation

Process(es)

protein binding MHC class II protein complex binding MHC class II receptor activity

HLA-DOA Polyclonal Antibody

Product Details

PA5-60904

Western Blot (WB)

Immunohistochemistry (IHC)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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