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Invitrogen
HMCES Polyclonal Antibody
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FIGURE: 1 / 3
HMCES Antibody (PA5-70309) in WB
Western blot analysis of HMCES was performed by loading whole cell lysate in 1X LDS sample buffer with 2-ME from 5 x 105 mouse transformed pre-B cells transduced with or without guide RNA targeting HMCES (gHMCES). Samples were loaded onto a 4-12 % Bis-Tris polyacrylamide gel (Product # NP0336BOX). Proteins were transferred to nitrocellulose membrane with wet/tank transfer. Membrane was blocked in 5% milk/TBST. The membrane was probed with a polyclonal anti-HMCES antibody (Product # PA5-70309) at a dilution of 1:1,000 in 2% milk/TBST at 4 d... View More
Product Details
PA5-70309
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
synthetic peptide directed towards the middle region of human HMCES (aa 41-90)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS with 2% sucrose
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This target displays homology in the following species: Cow: 93%; Dog: 86%; Horse: 93%; Human: 100%; Mouse: 93%; Pig: 79%; Rabbit: 100%; Rat: 93%
Target Information
Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites (PubMed:30554877, PubMed:31235915, PubMed:31235913). Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue (PubMed:30554877, PubMed:31235913). The HMCES DNA-protein cross-link is then degraded by the proteasome (PubMed:30554877). Promotes error-free repair of abasic sites by acting as a 'suicide' enzyme that is degraded, thereby protecting abasic sites from translesion synthesis (TLS) polymerases and endonucleases that are error-prone and would generate mutations and double-strand breaks (PubMed:30554877). Has preference for ssDNA, but can also accommodate double-stranded DNA with 3' or 5' overhang (dsDNA), and dsDNA-ssDNA 3' junction (PubMed:31235915, PubMed:31806351). Also involved in class switch recombination (CSR) in B-cells independently of the formation of a DNA-protein cross-link: acts by binding and protecting ssDNA overhangs to promote DNA double-strand break repair through the microhomology-mediated alternative-end-joining (Alt-EJ) pathway. Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Abasic site processing protein HMCES; Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein; ES cell-specific 5hmC-binding protein; Peptidase HMCES; putative peptidase SRAPD1; SRAP domain-containing protein 1; UPF0361 protein C3orf37 homolog
Gene Aliases: 8430410A17Rik; C85376; Hmces; Srap1; Srapd1
UniProt ID: (Mouse) Q8R1M0
Entrez Gene ID: (Mouse) 232210
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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