Immunofluorescent analysis of HMGA2 in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min, using a HMGA2 polyclonal antibody (Product # PA5-21320). Green: Primary antibody at a dilution of 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 µm.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 109 of HMGA2 (Uniprot ID#P52926)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay-dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-21320 targets HMGA2 in Immunohistchemistry (P), Immunofluorescence, Chromatin Immunoprecipitation and Western Blot applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-21320 immunogen is a recombinant fragment corresponding to a region within amino acids 1 and 109 of HMGA2 (Uniprot ID#P52926).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.