Invitrogen

HMGA2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (3) HMGA2 antibodies

Questions & Answers

Questions & Answers

Product Image Gallery

HMGA2 Antibody in Western Blot (WB) — Western blot was performed using Anti-HMGA2 Polyclonal Antibody (Product # PA5-25276) and a 18 kDa band corresponding to High mobility group protein HMGI-C was observed. Nuclear enriched extracts (30 µg lysate) of HCT 116 (Lane 1), SW480 (Lane 2), HT-29 (Lane 3), Hep G2 (Lane 4), ACHN (Lane 5), Caki-1 (Lane 6), A549 (Lane 7), A549 treated with 10 ng/mL TGF-B1 for 48 hrs (Lane 8) and NIH/3T3 (Lane 9) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Relative expression was observed between HCT 116 and HT-29. TGF-B1 induced upregulation of HMGA2 expression was also observed in A549.

HMGA2 Antibody in Western Blot (WB) — Western blot analysis using a HMGA2 polyclonal antibody (Product # PA5-25276) in HepG2 cell lysates (35 µg per lane).

HMGA2 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of HepG2 cells using a HMGA2 polyclonal antibody (Product # PA5-25276) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

HMGA2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across the cell lines tested owing to their inherent genetic constitution. Relative expression of High mobility group protein HMGI-C was observed in HCT 116 in comparison with HT-29 which expresses very low levels of HMGA2 (https://doi.org/10.1093/carcin/bgw029). using Anti-HMGA2 Polyclonal Antibody (Product # PA5-25276) in Western Blot. {RE}

HMGA2 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using HMGA2 Polyclonal Antibody (Product # PA5-25276), shows upregulation of HMGA2 expression in A549 cells treated with TGF-B1 (https://doi.org/10.3892/ijmm.2016.2705). {TM}

Product Details

PA5-25276

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Flow Cytometry (Flow)	1:10-1:50	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide between 64-92 amino acids from the C-terminal region of human HMGA2 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.45 mg/mL
Purification	Protein A, Antigen affinity chromatography
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2542776

Product Specific Information

This antibody is predicted to react with mouse based on sequence homology.

Target Information

HMGA2, previously known as HMGI-C, belongs to the group of high mobility chromosomal proteins that have the AT-hook DNA-binding motif. It is highly and ubiquitously expressed during embryogenesis. Chromosomal rearrangements of the HMGA2 gene and overexpression are frequently associated with benign and malignant tumors, respectively. Human and mouse HMGA2 shares 95% amino acid sequence identity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: High mobility group AT-hook protein 2; High mobility group protein HMGI-C; high mobility group protein I, isoform C; high-mobility group (nonhistone chromosomal) protein isoform I-C; High-mobility group protein HMGI-C

Gene Aliases: 9430083A20Rik; BABL; HMGA2; HMGI-C; HMGIC; LIPO; pg; pygmy; STQTL9

UniProt ID: (Human) P52926, (Mouse) P52927

Entrez Gene ID: (Human) 8091, (Mouse) 15364

Molecular Function: endodeoxyribonuclease

negative regulation of transcription from RNA polymerase II promoter epithelial to mesenchymal transition chondrocyte differentiation mesodermal-endodermal cell signaling base-excision repair chromatin organization regulation of transcription, DNA-templated transcription from RNA polymerase II promoter mitotic nuclear division mitotic G2 DNA damage checkpoint multicellular organism development response to virus regulation of cell cycle process positive regulation of gene expression chromosome condensation chromosome breakage heterochromatin assembly histone H2A-S139 phosphorylation senescence-associated heterochromatin focus assembly endodermal cell differentiation chondrocyte proliferation regulation of growth DNA damage response, detection of DNA damage positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of DNA binding negative regulation by host of viral transcription fat cell differentiation negative regulation of single stranded viral RNA replication via double stranded DNA intermediate negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter mesodermal cell differentiation mesenchymal cell differentiation stem cell differentiation cell division positive regulation of cell cycle arrest oncogene-induced cell senescence regulation of stem cell population maintenance positive regulation of stem cell proliferation positive regulation of transcription regulatory region DNA binding positive regulation of cellular response to X-ray positive regulation of cellular senescence positive regulation of response to DNA damage stimulus negative regulation of double-strand break repair via nonhomologous end joining regulation of cellular response to drug transcription, DNA-templated cell cycle signal transduction spermatogenesis cell proliferation positive regulation of cell proliferation male gonad development cell proliferation in forebrain pituitary gland development adrenal gland development negative regulation of intracellular steroid hormone receptor signaling pathway somatic stem cell population maintenance positive regulation of multicellular organism growth negative regulation of JAK-STAT cascade negative regulation of astrocyte differentiation positive regulation of sequence-specific DNA binding transcription factor activity meiotic cell cycle regulation of growth hormone secretion adipose tissue development fat pad development regulation of peptide hormone secretion nucleic acid phosphodiester bond hydrolysis

Process(es)

regulatory region DNA binding transcription factor activity, transcription factor binding core promoter binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding AT DNA binding DNA-(apurinic or apyrimidinic site) lyase activity DNA-dependent protein kinase activity protein binding transcription factor binding DNA binding, bending nucleosomal DNA binding cAMP response element binding MH2 domain binding MH1 domain binding SMAD binding 5'-deoxyribose-5-phosphate lyase activity C2H2 zinc finger domain binding enzyme binding