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Proteintech
GFP tag Monoclonal Antibody (1E10H7), HRP
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
HRP-66002
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
1E10H7
Immunogen
Recombinant protein
Conjugate
HRP
HRP
HRP
Form
Liquid
Concentration
1.33 mg/mL
Amount
133 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.5% BSA
Contains
0.02% thimerosal, 0.05% ProClin 300
Storage conditions
-20°C, store in dark
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
This antibody is conjugated with HRP. Note: Do not add azide (Sodium Azide or Smite) into the dilution buffer. azide is the HRP inhibitor which decreases the enzymatic activity of HRP.
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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